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ID   CLA12NCO   preliminary; circular DNA; SYN; 125 BP.
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AC   M17545;
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DT   15-JUN-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate plasmid vector CLA12NCO - incomplete, adaptor.
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KW   cloning vector; retrovirus; helper independent.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-125
RC   pUC12N from pUC12 & linker
RC   327AC from pBR327
RC   327ACR from 327AC
RC   327ACRH from 327ACR
RC   Cla12 from pUC12N & 327ACRH & pBR322
RC   Cla12Nco from Cla12 & pUC12N & L-src eukaryotic initiator
RC   SA Cla12Nco from Cla12 & pUC12N & pr779/1089-989/1079-2795/2795
RA   Hughes S.H., Greenhouse J.J., Petropoulos C.J., Sutrave P.;
RT   "Adaptor plasmids simplify the insertion of foreign DNA into
RT   helper-independent retroviral vectors";
RL   J. Virol. 61:3004-3012(1987).
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CC   NCBI gi: 209341
CC   NM (CLA12NCO)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC12 2680bp, at ATG
FT                   NcoI linker 6bp ccatgg
FT                   -> pUC12N 2680bp
FT                   1. RSV, src gene
FT                   -> L-src
FT                   1. Cla12 remove small ClaI-EcoRI 10bp 25..35, 1930bp
FT                   2. L-src ClaI-NcoI, eukaryotic initiator
FT                   3. pUC12N NcoI-EcoRI ?..231, MCS
FT                   -> Cla12Nco 2000bp"
FT   rep_origin      0..0
FT                   /note="ORI retrovirus"
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SQ   Sequence 125 BP; 27 A; 38 C; 35 G; 25 T; 0 other;
     atcgattcta gaccactgtg gccaggcggt agctgggacg tgcagccgac caccatggcc
     atgattacga attcgagctc gcccggggat cctctagagt cgacctgcag cccaagctta
     tcgat
//