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ID   COS202     preliminary; circular DNA; SYN; 10200 BP.
AC   ATCC37549;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli cosmid vector cos202 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   p202 from unpublished-plasmid & linker
RC   cos202 from p202 & pLTC
RC   cos203 from cos202 & pUC19
RA   Kioussis D., Wilson F., Daniels C., Leveton C., Taverne J.,
RA   Playfair J.H.;
RT   "Expression and rescuing of a cloned human tumour necrosis factor
RT   gene using an EBV-based shuttle cosmid vector";
RL   EMBO J. 6:355-361(1987).
RN   [2]
RC   unpublished-plasmid from pBR322 & hph gene & HSV tk prom & EBV EBNA-1
RA   Sugden B.;
RT   ;
RL   Unpublished (1987).
RN   [3]
RC   pLTC from lambda, cos
RA   Grosveld F.;
RT   ;
RL   Unpublished (1987).
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII--uncut; XhoI--10.05; BglII--10.05. (ATCC staff)
CC   The hygromycin resistance gene is expressed under the control of the
CC   HSV-TK promoter and polyadenylation signal. This is not an expression
CC   vector. (personal communication)
CC   A cosmid vector which can be selected in eukaryotic cells by
CC   hygromycin resistance.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (cos202)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pLTC)(p202 from EBNA-1 + hph + pBR322)
CC   BR ()
CC   OF (cos203)
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda, cos
FT                   -> pLTC
FT                   1. pBR322 4361bp
FT                   2. HSV, tk promoter/polyA/#X14112
FT                   3. hph gene
FT                   4. EBV EBNA-1 gene
FT                   -> plasmid
FT                   1. plasmid NruI, 5' to end of hph gene
FT                   BglII linker 6bp agatct
FT                   -> p202
FT                   1. p202 NarI, between EBV and hph gene
FT                   2. pLTC PvuII-NruI, lambda cos
FT                   -> cos202 10200bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO HSV thymidine kinase tk gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT vertebrate hygromycin phosphotransferase
FT                   gene; hygromycin resistance gene (hyg)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;