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ID   COS203     preliminary; circular DNA; SYN; 10200 BP.
AC   ATCC37550;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli cosmid vector cos203 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   p202 from unpublished-plasmid & linker
RC   cos202 from p202 & pLTC
RC   cos203 from cos202 & pUC19
RA   Kioussis D., Wilson F., Daniels C., Leveton C., Taverne J.,
RA   Playfair J.H.;
RT   "Expression and rescuing of a cloned human tumour necrosis factor
RT   gene using an EBV-based shuttle cosmid vector";
RL   EMBO J. 6:355-361(1987).
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII--10.05; XhoI--10.05; BglII--uncut. (ATCC staff)
CC   The cloning sites were derived from the M13mp10/pUC12 polylinker with
CC   the EcoRI site having been destroyed. (personal communication)
CC   The hygromycin resistance gene is expressed under the control of the
CC   HSV-TK promoter with a polyadenylation signal. This is not an
CC   expression vector. (personal communication)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (cos203)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (cos202)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda, cos
FT                   -> pLTC
FT                   1. pBR322 4361bp
FT                   2. HSV, tk promoter/polyA/#X14112
FT                   3. hph gene
FT                   4. EBV EBNA-1 gene
FT                   -> plasmid
FT                   1. plasmid NruI, 5' to end of hph gene
FT                   BglII linker 6bp agatct
FT                   -> p202
FT                   1. p202 NarI, between EBV and hph gene
FT                   2. pLTC PvuII-NruI, lambda cos
FT                   -> cos202 10200bp
FT                   1. cos202 BglII 10200bp
FT                   blunt end
FT                   2. pUC19 small EcoRI-HaeIII/FnuDI 6bp 391..397, MCS
FT                   \ HaeIII = 289 391 648 822 833 851 1285 1743 1823 2090
FT                   \ 2677
FT                   blunt end:blunt end
FT                   -> cos203 10200bp"
FT   misc_binding    0..0
FT                   /note="MCS SacI-SmaI-BamHI-XbaI-SalI-AccI-HincII-
FT                   PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI-SmaI-BamHI-XbaI-SalI-AccI-
FT                   HincII-PstI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO HSV thymidine kinase tk gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT vertebrate hygromycin phosphotransferase
FT                   gene; hygromycin resistance gene (hyg)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;