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ID   EBOPLPP    preliminary; circular DNA; SYN; 10572 BP.
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AC   ATCC37704;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector EBOpLPP - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   EBOpLPP from EBO-pcD- series & pUC19 & lambda, pL
RA   Canfield V., Emanuel J.R., Spickofsky N., Levenson R.,
RA   Margolskee R.F.;
RT   "Ouabain-resistant mutants of the rat Na,K-ATPase alpha 2 isoform
RT   identified by using an episomal expression vector";
RL   Mol. Cell. Biol. 10:1367-1372(1990).
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RN   [2]
RC   [from none]
RA   ;
RT   "DNA Protein Engineering Techniques";
RL   Meth. Mol. Cell. Biol. 2:0-0(1990).
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CC   This was also constructed from the SfiI fragment of EBOpSV2neo
CC   (hygromycin phosphotransferase gene, oriP, and EBNA-1 gene). [1]
CC   This is a shuttle vector permitting expression in primate cell lines.
CC   It is useful for subcloning. [2]
CC   This was constructed from the HindIII/EcoRI fragment of pcDV (pBR322
CC   ori, beta-lactamase, SV40 late polyadenylation signal) and the
CC   HindIII/PstI fragment of pL (SV40 early promoter and SV40 late
CC   intervening sequence). [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII/EcoRI--7.0, 3.5, 0.6; EcoRI--7.0, 4.0; SalI--10.6; XbaI--10.6.
CC   (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (EBOpLPP)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   HO (human cell line)(simian cell line)(dog cell line)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. EBO-pSV2neo SfiI 5729bp 3163..3163, MCS
FT                   \ oriP/hyg/EBNA-1
FT                   2. pUC19 SacI-KpnI-NotI-SalI-XbaI-HindIII 41bp
FT                   \ 407..448, MCS
FT                   3. pcDV1 HindIII-EcoRI 2537bp 3052..3053..2536,
FT                   \ ori/amp/SV40 late polyA
FT                   4. pL1 HindIII-PstI 2265bp,SV40 early prom/late intron
FT                   -> EBOpLPP 10572bp"
FT   misc_binding    0..0
FT                   /note="MCS KpnI-NotI-SalI-XbaI-HindIII-SacI"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI-NotI-SalI-XbaI-HindIII-SacI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT vertebrate hygromycin phosphotransferase
FT                   gene; hygromycin resistance gene (hyg)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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