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ID   EBOPSV2NEO preliminary; circular DNA; SYN; 13360 BP.
AC   ATCC37593;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector EBO-pSV2-neo - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   p220-1 from p201, EBV ori/EBNA-1/hph gene
RC   pcD-hprt from hprt & pcDV1 & pL1
RC   pcD-Leu-2 from T-cell surface marker Leu-2 & pcDV1 & pL1
RC   EBO-pSV2-neo from pSV2-neo & p220-1
RC   EBO-pcD-Leu-2 from pcD-Leu-2 & p220-1
RC   EBO-pcD-hprt from pcD-hprt & p220-1
RA   Margolskee R.F., Kavathas P., Berg P.;
RT   "Epstein-Barr virus shuttle vector for stable episomal replication
RT   of cDNA expression libraries in human cells";
RL   Mol. Cell. Biol. 8:2837-2847(1988).
RN   [2]
RC   SVGT7 from SV40
RA   Mulligan R.C., White R.T., Berg P.;
RT   "Formation of beta-globin following infection with recombinants
RT   containing rabbit beta-globin cDNA at different locations of
RT   SV40's late region";
RL   Advances in Gene Technology 0:201-215(1985).
RN   [3]
RC   pBR322SV or pSV1 from pBR322 & SV40
RC   pSV1betaG from pSV1 & beta-globin gene
RC   pSV1GT5, pSV1GT7 from SV40 & pSV1betaG
RC   pSV1GT5-gpt from pSV1GT5 & gpt gene
RC   pSV1GT7-gpt from pSV1GT7 & gpt gene
RC   pSV2-gpt from pSV2 & gpt gene
RC   from pSV2-gpt & linker
RA   Mulligan R.C., Berg P.;
RT   "Factors governing the expression of a bacterial gene in mammalian
RT   cells";
RL   Mol. Cell. Biol. 1:449-459(1981).
CC   The vector contains EBV oriP and EBNA to ensure replication as
CC   episomes in human or simian cells, even if the cell line is not EBNA
CC   positive. [1]
CC   pcD cDNA libraries can be converted into EBO-pcD libraries by ligating
CC   the 7.4 kb SfiI fragment of EBO-pSV2-neo  (containing oriP, EBNA-1,
CC   hph) into SfiI-linearized, phosphatased, pcD DNA. [1]
CC   A selectable, stably replicating shuttle vector for expression of
CC   cDNAs in eukaryotic cells; maintained episomally. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   NotI--uncut; BssHII--13.4; SfiI--7.5, 6.1; PstI--7.4, 2.3, 1.5, 1.0;
CC   SacI--13.4; BamHI--6.8, 5.4; HindIII--13.4; BglII--6.6. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (EBO-pSV2-neo)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli DH1)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. p201, EBV ori/EBNA-1/hph gene
FT                   -> p220-1
FT                   1. pSV2-neo SfiI, MCS
FT                   2. p220-1 BamHI-BamHI 7400bp, EBV ori/EBNA-1/hph gene
FT                   circularize
FT                   SfiI-SfiI 7400bp, EBV ori/EBNA-1
FT                   -> EBO-pSV2-neo"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
FT   promoter        0..0
FT                   /note="PRO SV40 late genes"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT mammal hygromycin phosphotransferase gene;
FT                   hygromycin resistance gene (hyg)"
FT   CDS             0..0
FT                   /note="ANT mammal G418 resistance gene (G418)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;