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ID   EMBL3AMBM  preliminary; circular DNA; SYN; 44000 BP.
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AC   ATCC37268;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda EMBL3 AamBam - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   EMBL1 from lambda 1059 & pBR322
RC   EMBL2 from EMBL1
RC   EMBL2Sal, EMBL2RI from EMBL2
RC   [pUC2 from M13mp2]
RC   pUC(S-Bg), pUC(R-Bg) from pUC2
RC   EMBL3 from EMBL2Sal & EMBL2RI & pUC(S-Bg)
RC   EMBL4 from EMBL2Sal & EMBL2RI & pUC(R-Bg)
RC   EMBL3AamBam, EMBL3Sam7, EMBL3Aam, EMBL3Bam from EMBL3
RC   EMBL3AamSam, EMBL3BamSam from EMBL3
RA   Frischauf A.M., Lehrach H., Poustka A., Murray N.E.;
RT   "Lambda replacement vectors carrying polylinker sequences";
RL   J. Mol. Biol. 170:827-842(1983).
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CC   Partial genotype: Aam Bam.
CC   A lambda replacement vector for construction and screening of genomic
CC   libraries.
CC   Phage with inserts have the Spi- phenotype.
CC   Medium is 1592 SM buffer.
CC   NM (EMBL3 AamBam)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteria-free lysate)(E.coli)(E.coli NM538)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Charon 4A left end-AvaI 4721bp 1..4721,
FT                   \ Aam32 Bam1
FT                   2. EMBL3 AvaI-KpnI 18822bp 4721..23543, middle
FT                   3. EMBL3 KpnI-right end 5694bp 23543..29237
FT                   phosphatase
FT                   -> EMBL3AamBam 29237bp"
FT   misc_binding    0..0
FT                   /note="MCS SalI-PstI-BamHI-EcoRI-EcoRI-BamHI-PstI-
FT                   SalI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI-BamHI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   CDS             0..0
FT                   /note="GEN E. coli Spi+ reporter gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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