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ID   EMBL3COSNT preliminary; circular DNA; SYN; 48200 BP.
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AC   ATCC37603;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda EMBL3-cos-Not - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   mpAC5 from pWP14 & M13mp8
RC   [lambda NM1265, lambda NM1266 from lambda]
RC   lambda NM1274 from lambda NM1265 & mpAC5 & lambda NM1266
RC   lambda NM1275 from lambda NM1274 & EMBL3
RC   EMBL3cos from lambda NM1275
RC   lambda NM1277 from lambda & EMBL301
RC   lambda NM1278 from EMBL3cos & lambda NM1277
RC   EMBL3cos-Not from EMBL3cos & lambda NM1278
RA   Whittaker P.A., Campbell A.J., Southern E.M., Murray N.E.;
RT   "Enhanced recovery and restriction mapping of DNA fragments cloned
RT   in a new lambda vector";
RL   Nucleic Acids Res. 16:6725-6736(1988).
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CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--33.5, 14.5; BamHI--33.5, 14.5. (ATCC staff)
CC   For library construction, a double digest of the vector with BamHI +
CC   EcoRI and removal of the linker fragment reduces the number of
CC   background phage.
CC   Phosphatase treatment of digested vector eliminates background.
CC   (personal communication)
CC   By labelling the left cohesive end, which is 200 bp from cloning
CC   sites, reliable restriction maps of clones can be made from partial
CC   digests. (personal communication)
CC   A modified EMBL vector, with all phage coding sequences to the right
CC   of the cloning sites to facilitate restriction mapping of clones. Can
CC   be used for genomic library construction. (personal communication)
CC   Medium is 1592 SM buffer.
CC   NM (EMBL3-cos-Not)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteria-free lysate)(E.coli MC1061)(E.coli K802)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M13mp18 7249bp
FT                   2. oligo
FT                   -> M13-sn-19 7300bp
FT                   1. EMBL3 29237bp
FT                   2. M13-sn-19 7300bp
FT                   -> EMBL301 36500bp
FT                   1. stuffer with no BglII EcoRI-EcoRI
FT                   2. EMBL301 EcoRI 36500bp 20067..20067
FT                   -> lambda NM1277
FT                   1. EMBL3cos BamHI 48200bp 20063..20063
FT                   2. lambda NM1277 BglII-BglII 22426..?, MCS/stuffer/MCS
FT                   -> lambda NM1278
FT                   1. EMBL3cos left end-EcoRI 20618bp 1..20618,
FT                   \ left arm/center
FT                   2. lambda NM1278 EcoRI-right end 20067..?, rightarm
FT                   -> EMBL3cos-Not 48200bp"
FT   misc_binding    0..0
FT                   /note="MCS PstI-SalI-BamHI-SmaI-EcoRI; EcoRI-BamHI-
FT                   SfiI-NaeI-NotI-SalI"
FT   misc_binding    0..0
FT                   /note="SIT double BamHI-EcoRI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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