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ID   F1P        preliminary; circular DNA; SYN; 254 BP.
AC   M10650;
DT   15-JUN-1988 (Rel. 3, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli phage vector f1' - incomplete, gene IV.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-254
RC   f1' from phage f1 & pSC101
RA   Ravetch J.V., Ohsumi M., Model P., Vovis G.F., Fischhoff D.,
RA   Zinder N.D.;
RT   "Organization of a hybrid between phage f1 and plasmid pSC101";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:2195-2198(1979).
RN   [2]
RC   lambda h80dhis from lambda & Salmonella hisOGD region
RC   pWB91 from pML21 & lambda h80dhis, Salmonella hisOGD region
RC   M13Hol67, M13Hol68, M13Hol76 from M13 & lambda h80dhis, hisOGD region
RA   Barnes W.M.;
RT   "Construction of an M13 histidine-transducing phage: a
RT   single-stranded cloning vehicle with one EcoRI site";
RL   Gene 5:127-139(1979).
RN   [3]
RC   VO-1 from phage f1 & pSC101
RC   f1' from VO-1
RA   Ohsumi M., Vovis G.F., Zinder N.D.;
RT   "The isolation and characterization of an in vivo recombinant
RT   between the filamentous bacteriophage f1 and the plasmid pSC101";
RL   Virology 89:438-449(1978).
CC   NCBI gi: 209158
CC   NM (f1')
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (phage f1)(pSC101)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. phage f1
FT                   recombination
FT                   2. pSC101, tet gene
FT                   -> phage VO-1
FT                   1. phage VO-1 remove, pSC101 except 200bp duplication
FT                   -> f1' [200bp larger than f1 at insertion site]"
FT   CDS             <1..33
FT                   /note="GEN bacteriophage f1' gene IV peptide;
FT                   NCBI gi: 209159"
SQ   Sequence 254 BP; 74 A; 57 C; 56 G; 67 T; 0 other;
     gtgctcgtca aagcaaccat agtaggggtc tgagggccaa tggaacgaaa acgtacgtta
     gtgaagtaac tgtctgatat atcgaaacat aatgtacatt ggaaaacgcc atcaaaacgg
     tgtcttttaa tcgaaattgg cactaacgga cttctgcaca atcgattttt cagacgataa
     ttttcctctt accgtacgtt ttcgtacggt tggacctcaa acccctagta cgcgccctgt
     agcggcgcat taag