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ID   FLACZCI857 preliminary; circular DNA; SYN; 1 BP.
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AC   ATCC37446;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector F'lacZ+cI857 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBR322-lac22 from pBR322 & F' lac22 A4, lacZ gene
RC   pEM1 from pBR322-lac22
RC   F' lac22 A4 cI+ from F' lac22 A4 & lambda
RC   F'lacZ+cI857 from F' lac22 A4 cI+ & pEM1
RC   pRM12 from pRM1 & gltA gene
RA   Mieschendahl M., Muller-Hill B.;
RT   "F'-coded, temperature-sensitive lambda cI857 repressor gene for
RT   easy construction and regulation of lambda promoter-dependent
RT   expression systems";
RL   J. Bacteriol. 164:1366-1369(1985).
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RN   [2]
RC   F' pro+ trp+ from F' factor
RC   F' lac- U118 pro+ or F' lac22 A4 from F' factor
RC   lambda cIts857 plac5 from lambda
RC   chromosome from lambda cIts857 plac5 & E. coli lacZ gene
RC   chromosome2 from chromosome & F' lac- U118 pro+ or F' lac22 A4
RC   DC20-2-22 from chromosome2 & F' pro+ trp+
RA   Mieschendahl M., Griesser H.W., Muller-Hill B.;
RT   "Lambda immunity phase shift in a lambda N--lacZ+ fusion";
RL   Mol. Gen. Genet. 183:202-204(1981).
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RN   [3]
RC   pRM1 from lambda, pL
RA   Mieschendahl M.;
RT   ;
RL   Unpublished (1985).
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CC   Facilitates construction and regulation of lambda promoter-dependent
CC   expression systems without using defective prophages.
CC   Medium is 1065 LB medium.
CC   NM (F'lacZ+cI857)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli DP90cNal)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. F' factor
FT                   2. E. coli lac gene
FT                   -> F' lac22 A4
FT                   1. F' lac22 A4 remove cIts857 gene
FT                   recombination
FT                   2. lambda, cI gene
FT                   -> F' lac22 A4 cI+
FT                   1. pBR322 EcoRI 4361bp 4360..4360
FT                   2. F' lac22 A4 EcoRI-EcoRI, lambda cII/cI857/rex/lac22
FT                   -> pBR322-lac22
FT                   1. pBR322-lac22 remove BglII-BglII 711bp 38103..38814,
FT                   \ cro
FT                   -> pEM1 [BglII-BglII 2392bp 35711..38103, cI857 gene]
FT                   1. F' lac22 A4 cI+ remove cI gene
FT                   recombination
FT                   2. pEM1, cI857 gene
FT                   -> F'lacZ+cI857"
FT   rep_origin      0..0
FT                   /note="ORI E. coli F factor"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PL"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PR"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli nalR gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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