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ID   L8136      preliminary; circular DNA; SYN; 130 BP.
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AC   M32717;
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DT   15-JUN-1990 (Rel. 5, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector L8-136 - incomplete, pBR322/T5.
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KW   cloning vector; transposon T5.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-130
RC   L8-136, L8-16 from L8
RC   from L51
RA   Brunel F., Heusterspreute M., Merchez M., Thi V.H., Pilaete M.F.,
RA   Davison J.;
RT   "Gene rearrangements leading to the expression of an
RT   insertion-inactivated tetracycline resistance gene in pBR322";
RL   Plasmid 9:201-214(1983).
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CC   NCBI gi: 208302
CC   NM (L8-136)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII
FT                   2. phage T5 HindIII-HindIII 3900bp, HindIII L frag
FT                   -> L8
FT                   1. L8
FT                   duplication
FT                   -> L8-136"
FT   misc_feature    42..42
FT                   /note="RNA I start"
FT   misc_recomb     117..118
FT                   /note="pBR322 DNA end/T5 DNA start"
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SQ   Sequence 130 BP; 33 A; 27 C; 35 G; 35 T; 0 other;
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaactat
     ctggcagatg
//