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ID   L816       preliminary; circular DNA; SYN; 164 BP.
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AC   M32718;
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DT   15-JUN-1990 (Rel. 5, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E. coli plasmid vector L8-16 - incomplete, pBR322/T5.
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KW   cloning vector; transposon T5.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-164
RC   [L8 from pBR322 & phage T5]
RC   [L51 from pBR322 & phage T5]
RC   L8-136, L8-16 from L8
RC   from L51
RA   Brunel F., Heusterspreute M., Merchez M., Thi V.H., Pilaete M.F.,
RA   Davison J.;
RT   "Gene rearrangements leading to the expression of an
RT   insertion-inactivated tetracycline resistance gene in pBR322";
RL   Plasmid 9:201-214(1983).
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RN   [2]
RC   lambda gtWES.T5-14 from lambda gtWES.lambdaB & T5
RC   from lambda gtWES::T5 series
RC   from pBR322::T5 series
RA   Brunel F., Davison J., Ha Thi V., Reeve J.;
RT   "Cloning of bacteriophage T5 DNA fragments III. Expression in E.
RT   coli mini-cells";
RL   Gene 16:107-118(1981).
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RN   [3]
RC   lambda gtWES::T5 series from lambda gtWES.lambdaB & T5
RC   pBR322::T5 series from pBR322 & T5
RA   Brunel F., Davison J., Merchez M.;
RT   "Cloning of bacteriophage T5 DNA fragments in plasmid pBR322 and
RT   bacteriophage lambda gtWES";
RL   Gene 8:53-68(1979).
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CC   NCBI gi: 208303
CC   NM (L8-16)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII
FT                   2. phage T5 HindIII-HindIII 3900bp, HindIII L frag
FT                   -> L8
FT                   1. L8 remove, amp gene
FT                   spontaneous deletion
FT                   -> L8-16"
FT   misc_feature    42..42
FT                   /note="RNA I start"
FT   misc_recomb     150..151
FT                   /note="pBR322 DNA end/T5 DNA start"
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SQ   Sequence 164 BP; 41 A; 35 C; 42 G; 46 T; 0 other;
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     ccaccgctgg tagcggtggt ttttttgttt tatagcacag caac
//