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ID   LAM1059    preliminary; circular DNA; SYN; 45800 BP.
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AC   ATCC37474;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phasmid phage vector lambda 1059 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pacI29 from ColE1 & lambda, att sites
RC   lambda 983 from lambda & pacI29
RC   lambda 1059 from pacI29 & lambda 983 & lambda, immunity region
RA   Karn J.M., Brenner S., Barnett L., Cesareni G.;
RT   "Novel bacteriophage lambda cloning vector";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:5172-5176(1980).
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RN   [2]
RC   pACL29, pACL30, pACL33, pACL47, pACL46 from lambda & RSF1050
RA   Brenner S., Cesareni G., Karn J.M.;
RT   "Phasmids: hybrids between ColE1 plasmids and E. coli bacteriophage
RT   lambda";
RL   Gene 17:27-44(1982).
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CC   The vector is Spi+; recombinants are Spi-.
CC   Replacement vector useful for library construction which accepts
CC   inserts of 6-24 kb.
CC   Medium is 1592 SM buffer.
CC   NM (lambda 1059)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteria-free lysate)(E.coli)
CC   HO (E.coli Q358 [for propagation])(E.coli Q359 [for Spi selection])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. ColE1 6646bp
FT                   2. lambda 500bp 27500..28000, att sites
FT                   \ [core 27723..27738]
FT                   -> pacI29 7100bp
FT                   1. pacI29 BamHI 7100bp, lambda 27973..27973
FT                   2. lambda BglII-BglII 32bp 738..780, immunity region
FT                   \ lambda 60bp 38755..38815
FT                   \ lambda BglII 35712 38104 38755 38815
FT                   \ lambda immunity region 34000..39000
FT                   -> plasmid 7100bp
FT                   1. plasmid 7100bp
FT                   insertion
FT                   2. phage 434 2662bp, imm434/lambda 35583..38245
FT                   3. lambda
FT                   -> lambda hlambda deltasR1 placsR1 lambda2 cI857
FT                   \ pacI29 delta{int-cIII} 434 cII chi153 Pam902
FT                   1. lambda hlambda deltasR1 placsR1 lambda2 cI857
FT                   \ pacI29 delta{int-cIII} 434 cII chi153 Pam902, right
FT                   2. lambda h80 delta{att80 - lambdacIII}, left arm
FT                   -> lambda h80 delta{att80 - lambdacIII} cI857
FT                   \ pacI29 P.P' delta{int-cIII} 434 cII chi153 Pam902
FT                   1. lambda h80 delta{att80 - lambdacIII} cI857
FT                   \ pacI29 P.P' delta{int-cIII} 434 cII chi153 Pam902,
FT                   \ left
FT                   2. lambda b1319 81 cIts chi3, right arm
FT                   -> lambda h80 delta{att80 - lambdacIII} cI857
FT                   \ pacI29 P.P' b1319 81 cIts chi3
FT                   1. lambda h80 delta{att80 - lambdacIII} cI857
FT                   \ pacI29 P.P' b1319 81 cIts chi3, left arm
FT                   2. lambda sBam 1o b189 int29 ninL44 cI857, right arm
FT                   -> lambda sBam 1o b189 int29 ninL44 cI857
FT                   \ pacI29 P.P' b1319 81 cIts chi3
FT                   1. lambda sBam 1o b189 int29 ninL44 cI857
FT                   \ pacI29 P.P' b1319 81 cIts chi3, left arm
FT                   2. lambda sBam 1o b189 pacI29  delta{int-cIII}
FT                   \ KH54 nin5 chi3, right arm
FT                   -> lambda 1059 45800bp
FT                   \ [hlambda sBam 1o b189 int29 ninL44
FT                   \ cI857 pacI29 delta{int-cIII} KH54 sR1 4o nin5 chi3]"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   CDS             0..0
FT                   /note="GEN E. coli Spi+ reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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