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ID   LAM2001    preliminary; circular DNA; SYN; 41900 BP.
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AC   ATCC37331;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda 2001 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   M13mp12 from M13mp8
RC   [lambda 1129 from lambda]
RC   [lambda 1277 from lambda]
RC   [lambda 1321 from lambda]
RC   [lambda 1543 from lambda]
RC   [lambda 2188 from lambda]
RC   lambda 1341 from lambda 983 & lambda 1321
RC   lambda 1672 from lambda 1543 & lambda 1321
RC   lambda 2004 from lambda 1672 & Charon 4A & lambda 1129
RC   lambda 1259 from lambda 1059
RC   lambda 1274 from lambda 1059
RC   lambda 2053 from lambda 2004
RC   lambda 2522 from lambda 2053 & M13mp12
RC   lambda 2149 from lambda 1059
RC   lambda 2537 from lambda 2522 & lambda vir & lambda 2188
RC   lambda 2001 from lambda 2537 & lambda 1277
RA   Karn J.M., Matthes H.W., Gait M.J., Brenner S.;
RT   "A new selective phage cloning vector, lambda 2001, with sites for
RT   XbaI, BamHI, HindIII, EcoRI, SstI and XhoI";
RL   Gene 32:217-224(1984).
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CC   Rehydrate in 1 ml 10 mM magnesium sulphate.
CC   Recombinants are Spi- cI- Gam- Int- Red-.
CC   A lambda replacement vector carrying a polylinker sequence with
CC   positive selection for large (10-23 kb) inserts.)
CC   Genotype of vector is lambdasbhI lambda1(0)b189 SIGMAint (linker)
CC   srIlambda3(0) ninL44 SIGMAshndIII lambda4 (bio) (linker)
CC   delta(sbhIlambda3-sbhIlambda4) KH54 srIlambda4(0) chiC nin5
CC   srIlambda5(0) shndIIIlambda6(0).
CC   The arms are 20.1 kb and 9.4 kb. (ATCC staff)
CC   Medium is 1592 SM buffer.
CC   NM (lambda 2001)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteria-free lysate)(E.coli)
CC   HO (E.coli LE392 [for propagation])(E.coli NM539 [for recombinant])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda 1059 45800bp
FT                   \ [lambda b189 deltaBam KH54 nin5]
FT                   1. lambda
FT                   -> lambda 1129
FT                   \ [lambda deltaBam cI857 chiC nin5]
FT                   1. lambda
FT                   -> lambda 1321
FT                   \ [lambda b189 pACL29 deltaatt80 KH54 chiC nin5]
FT                   1. lambda
FT                   -> lambda 1543
FT                   \ [lambda b189 int29 ninL44 cI857 chiC]
FT                   1. lambda 1321
FT                   2. lambda 1543
FT                   -> lambda 1672
FT                   \ [lambda b189attint29ninL44cI857deltaBamKH54chiCnin5]
FT                   1. lambda 1672 end-HindIII
FT                   :AMV polymerase
FT                   2. Charon 4A EcoRI-EcoRI, bio256
FT                   AMV polymerase:AMV polymerase
FT                   BamHI-BamHI
FT                   EcoRI-BamHI, bio256
FT                   3. lambda 1129 BamHI-end
FT                   -> lambda 2004
FT                   1. lambda 2004 BamHI
FT                   XbaI linker 10bp gatctctaga
FT                   BamHI
FT                   XbaI linker 10bp gatctctaga
FT                   -> lambda 2053
FT                   1. lambda
FT                   -> lambda 2188
FT                   \ [lambda phi80att80 tonBtrpD'deltaBam virPam1001nin5]
FT                   1. lambda 2053 XbaI
FT                   2. M13mp8, MCS
FT                   -> lambda 2522
FT                   1. lambda
FT                   -> lambda 1277
FT                   \ [lambda phi80att80tonBtrpD' deltaBam KH54 chiC nin5]
FT                   1. lambda 2522
FT                   2. lambda 2188
FT                   -> lambda 2537
FT                   \ [lambda b189 att ninL44 deltaBam vir Pam1001 nin5]
FT                   1. lambda 2537
FT                   cross with
FT                   2. lambda 1277
FT                   -> lambda 2001 41900bp
FT                   \ [lambda b189bio256att ninL deltaBam KH54chiCnin5]"
FT   misc_binding    0..0
FT                   /note="MCS SstI-XhoI-BamHI-HindIII-EcoRI-XbaI"
FT   misc_binding    0..0
FT                   /note="SIT unique SstI-XhoI-BamHI-HindIII-EcoRI-XbaI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   CDS             0..0
FT                   /note="GEN E. coli Spi+ reporter gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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