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ID   LAMAMP3    preliminary; circular DNA; SYN; 1 BP.
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AC   ATCC37230;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda amp3 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   lambda amp3 from lambda gt11 & pBR322 & linker
RA   Kemp D.J., Coppel R.L., Cowman A.F., Saint R., Brown G.V.,
RA   Anders R.F.;
RT   "Expression of Plasmodium falciparum blood-stage antigens in
RT   Escherichia coli: detection with antibodies from immune humans";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:3787-3791(1983).
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CC   Temperature sensitive.
CC   A lambda expression vector that permits insertion of foreign DNA into
CC   the lacZ gene and promotes regulated synthesis of hybrid proteins.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (lambda amp3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (E.coli W3110)(E.coli)(E.coli Y1088)(E.coli Y1089)(E.coli Y1090)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda gt11 XbaI 43700bp,
FT                   \ lambda 24509..24509
FT                   2. pBR322 BstNI/EcoRII-EcoRI 1726bp 2634..4360, amp
FT                   BAL31 nuclease
FT                   XbaI linker 6bp tctaga:XbaI linker 6bp tctaga
FT                   XbaI-XbaI 1700bp, amp gene
FT                   -> lambda amp3 45400bp"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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