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ID   LAMCIKH100 preliminary; circular DNA; SYN; 1 BP.
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AC   ATCC40119;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda cIKH100 (IS5) - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [lambda Nam7 Nam53 cIts857 from lambda]
RC   lambda cIKH100 (IS5) from lambda Nam7 Nam53 cIts857 & IS5
RA   ;
RT   ;
RL   Unpublished (1988).
RL   U.S. Patent No. 4,774,182 dated Sept. 27, 1988.
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CC   This was isolated as spontaneous mutant of a lambda Nam7 Nam53 cIts857
CC   prophage.
CC   This replicates in both sup and sup0 E.coli hosts and forms
CC   clear plaques at both 30 and 42C. (personal communication)
CC   This is bacteriophage lambda with an IS5 insertion in gene cI.
CC   Medium is 1592 SM buffer.
CC   NM (lambda cIKH100 IS5)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteria-free lysate)(E.coli)(E.coli C600)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda Nam7 Nam53 cIts857
FT                   1. lambda Nam7 Nam53 cIts857
FT                   mutation
FT                   -> lambda cIKH100 (IS5) [IS5 in cIts857]"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PL"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PR"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//