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ID   LAMDR2     preliminary; circular DNA; SYN; 45700 BP.
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AC   IG1007;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda DR2 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   phage from lambda ZD35
RC   lambda Zd35B from phage & rpZd5
RC   rpZd6 from rpZd5 & LNL6
RC   rpZd6deltaRI from rpZd6
RC   rpZd7, rpZd8 from rpZd6deltaRI & oligo
RC   lambda Zd37 from lambda Zd35B & rpZd7
RC   plambda Zd39 from lambda Zd37 & pEA305, tac promoter/lambda repressor
RA   Murphy A.J., Schimke R.T.;
RT   "p lambda Zd39: a new type of cDNA expression vector for low
RT   background, high efficiency directional cloning";
RL   Nucleic Acids Res. 19:3403-3408(1991).
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RN   [2]
RC   lambda DR2 from lambda
RC   pDR2 from lambda
RA   Murphy A.J., Kung A.L., Swirski R.A., Schimke R.T.;
RT   ;
RL   Unpublished (1992).
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CC   NM (lambda DR2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (CLONTECH)
CC   HO (E.coli)(human fibroblast or lymphoblast cells)
CC   CP ()
CC   FN (cloning cDNA)
CC   SE ()
CC   PA (lambda)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda, cI gene/loxP site
FT                   2. EBV, EBNA-1 gene
FT                   3. RSV, LTR
FT                   4. SV40 HindIII-PvuII 344bp 5172..5243..273,
FT                   \ early region
FT                   5. HSV, tk gene/#X14112
FT                   6. E. coli 1142bp, hyg gene
FT                   -> lambda DR2 45700bp"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda loxP"
FT   misc_binding    0..0
FT                   /note="SIT ClaI"
FT   CDS             0..0
FT                   /note="GEN Epstein-Barr virus nuclear antigen gene
FT                   (EBNA-1)"
FT   rep_origin      0..0
FT                   /note="ORI Epstein-Barr virus (EBV) oriP"
FT   promoter        0..0
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT XboI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA mammal thymidine kinase (TK) gene"
FT   CDS             complement(0..0)
FT                   /note="ANT mammal hygromycin phosphotransferase gene;
FT                   hygromycin resistance gene (hyg)"
FT   promoter        complement(0..0)
FT                   /note="PRO mammal thymidine kinase (TK) gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT ClaI"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda loxP"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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