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ID   LAMEXCELL  preliminary; circular DNA; SYN; 45500 BP.
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AC   IG1008;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda ExCell - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   lambda ExCell from lambda
RA   ;
RT   ;
RL   Unpublished (1992).
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CC   NM (lambda ExCell)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (Pharmacia)
CC   HO (E.coli NM522)(E.coli NP66)
CC   CP ()
CC   FN (cloning up to 6000 bp)(cloning directional)
CC   FN (cloning cDNA)(transcription)
CC   SE (color blue/white)
CC   PA (Charon)
CC   BR (pExCell)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda, cos
FT                   2. f1, ori
FT                   3. T7, promoter
FT                   4. Sp6, promoter
FT                   5. E. coli, lacZ gene
FT                   -> lambda ExCell 45500bp"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosL)"
FT   misc_feature    0..0
FT                   /note="SIT bacteriophage lambda attR"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII (in C-terminus of lacZ)"
FT   misc_binding    0..0
FT                   /note="SIT unique MluI (in C-terminus of lacZ)"
FT   misc_binding    0..0
FT                   /note="SIT unique NotI (in C-terminus of lacZ)"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI (in C-terminus of lacZ)"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI (in C-terminus of lacZ)"
FT   misc_binding    0..0
FT                   /note="SIT unique SfiI (in C-terminus of lacZ)"
FT   misc_binding    0..0
FT                   /note="SIT unique XhoI (in C-terminus of lacZ)"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage Sp6"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    0..0
FT                   /note="SIT bacteriophage lambda attL"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda phi80 imm"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosR)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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