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ID   LAMEXLX    preliminary; circular DNA; SYN; 64 BP.
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AC   M54945;
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DT   18-JAN-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda EXLX - incomplete, promoter.
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KW   cloning vector; lambda EXLX.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-64
RC   pGLX2 from pZf18R & oligo
RC   lambda GLX from lambda gt10 & pGLX2, loxP
RC   pGLX1 from lambda GLX
RC   [lambda NM1150 from lambda]
RC   lambda PH1 from lambda NM1150 & lambda JP3
RC   lambda PH2 from lambda PH1 & pGLX2
RC   lambda PH3 from lambda PH2 & pC4beta-gal
RC   lambda LOX from lambda PH3
RC   pMP1+ from pGEM-3Zf+ & oligo
RC   pMP1- from pGEM-3Zf- & oligo
RC   pMP2+ from pMP1+ & pET-3xa
RC   pMP2- from pMP1- & pET-3xa
RC   pMP3+ from pMP2+ & oligo
RC   pMP3- from pMP2- & oligo
RC   lambda EXLX+ from pMP3+ & lambda LOX
RC   lambda EXLX- from pMP3- & lambda LOX
RC   pDD1, pDD2 from pCM1 & oligos
RC   lambda SHLX1 from pDD1 & lambda LOX
RC   lambda SHLX2 from pDD2 & lambda LOX
RA   Palazzolo M.J., Hamilton B.A., Ding D., Martin C.H., Mead D.A.,
RA   Mierendorf R.C., Raghavan K.V., Meyerowitz E.M., Lipshitz H.D.;
RT   "Phage lambda cDNA cloning vectors for subtractive hybridization,
RT   fusion-protein synthesis and Cre-loxP automatic plasmid subcloning";
RL   Gene 88:25-36(1990).
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RN   [2]
RC   lambda 1150 from lambda
RA   Murray N.;
RT   "Lambda vectors";
RL   Lambda II 0:677-684(1983).
RL   Hendrix R.W., Roberts J.W., Stahl F.W., Weisberg R.A.;
RL   Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
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RN   [3]
RC   lambda JP3 from lambda NM1150
RA   Strathmann M., Simon M.;
RT   ;
RL   Unpublished (1983).
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RN   [4]
RC   pCM1 from pGEM-3Zf+ & pATH1
RA   Martin C.H., Mayeda C., Palazzolo M.J.;
RT   ;
RL   Unpublished (1990).
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RN   [5]
RC   pCGE41 from pMLB1034 & linker
RA   Goff C.;
RT   ;
RL   Unpublished (1988).
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RN   [6]
RC   pDmA2 from act5 gene
RA   Fyrberg E.A., Bond B.J., Hershey N.D., Mixter K.S., Davidson N.;
RT   "The actin genes of Drosophila: protein coding regions are highly
RT   conserved but intron positions are not";
RL   Cell 24:107-116(1981).
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RN   [7]
RC   pAMG12 from act5 operon
RA   Price D., Parker C.;
RT   ;
RL   Unpublished (1988).
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CC   NCBI gi: 208749
CC   NM (lambda EXLX)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM3Zf+ remove PvuII-PvuII 157bp
FT                   \ 3090..3199..27, lac/MCS/3063bp
FT                   2. oligo HincII-HindIII-BglII-SmaI 40bp
FT                   -> pML1+ 3100bp
FT                   1. pML1+ remove small BglII-HincII, 3100bp
FT                   2. pET3xa small BglII-EcoRV, T7 gene 10/terminator
FT                   -> pML2+ 3100bp
FT                   1. lambda
FT                   -> lambda NM1150
FT                   1. lambda NM1150 ApaI, lambda 10091..10091
FT                   fill in
FT                   remove small EcoRI-HindIII
FT                   2. E. coli EcoRI-HindIII 650bp, amp gene
FT                   -> m lambda J
FT                   1. pML2+ 3100bp
FT                   recombination
FT                   2. m lambda J
FT                   -> m lambda MP2+
FT                   1. m lambda MP2+ BamHI
FT                   2. oligo HindIII-XbaI-EcoRI
FT                   -> m lambda MP2P [no BamHI]
FT                   1. m lambda MP2P remove HindIII-EcoRI
FT                   2. oligo SacI-HindIII-ApaI-Sp6 promoter site
FT                   -> phage [no original HindIII]
FT                   1. phage
FT                   -> pMP3+
FT                   1. lambda NM1150 ApaI, lambda 10091..10091
FT                   fill in
FT                   make ApaI in right arm
FT                   -> m lambda JP3
FT                   1. lambda NM1150 SnaBI-right end 36311bp,
FT                   \ lambda 12191..48502
FT                   2. m lambda JP3 left end-SnaBI 12191bp,
FT                   \ lambda 0..12191
FT                   -> lambda PH1 48502bp
FT                   1. pZF18R SmaI 2900bp, MCS
FT                   \ pTZ18R SmaI 2861bp 200..200
FT                   2. oligo loxP-SmaI-loxP 74bp [34+6+34]
FT                   -> pGLX2 3000bp
FT                   1. lambda PH1 XhoI 48502bp, lambda 33499..33499
FT                   2. pGLX2 XhoI-XhoI 100bp, loxP sites
FT                   -> lambda PH2 48500bp
FT                   1. pSV2-dhfr 5000bp
FT                   2. Carnegie 4 3630bp
FT                   -> plasmid 8600bp
FT                   1. pMLB1034 6189bp
FT                   linker
FT                   -> pCGE41 6200bp
FT                   1. plasmid 8600bp
FT                   2. pCGE41, lacZ gene/pMLB1034 3000..6000
FT                   -> pC4beta-gal
FT                   1. lambda PH2 remove SacI-XbaI, pGLX2
FT                   2. pC4beta-gal SacI-XbaI 850bp, SV40 sequence
FT                   -> lambda PH3
FT                   1. lambda PH3 EcoRI-HindIII
FT                   Klenow:Klenow
FT                   -> lambda LOX
FT                   1. pMP3+ SmaI
FT                   2. lambda LOX SacI-XbaI
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> lambda EXLX+"
FT   rep_origin      0..0
FT                   /note="ORI"
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SQ   Sequence 64 BP; 16 A; 16 C; 13 G; 19 T; 0 other;
     ggatcgaatt cgagctcaag cttgggccct attctatagt gtcacctaaa tactagcttg
     atcc
//