back Return to this vector's summary.
ID   LAMGT10    preliminary; circular DNA; SYN; 43340 BP.
XX
AC   U02447; IG0303; ATCC40179;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector lambda gt10 - incomplete, near cloning site.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   from lambda gt10, cloning methods
RC   from lambda gt11, cloning methods
RA   Huynh T.V., Young R.A., Davis R.W.;
RT   "Constructing and screening cDNA libraries in lambda gt10 and
RT   lambda gt11";
RL   DNA Cloning 1:49-78(1985).
RL   Glover D.M., ed., IRL Press, Oxford.
XX
RN   [2]
RC   from lambda gt10
RC   from lambda gt11
RA   Jendrisak J., Young R.A., Engel J.D.;
RT   "Cloning cDNA into lambda gt10 and lambda gt11";
RL   Meth. Enzymol. 152:359-371(1987).
XX
RN   [3]
RC   [lambda 569 from lambda]
RC   lambda 590, lambda 728, lambda 607 from lambda imm434
RC   lambda 598 from lambda 590
RC   lambda 641 from lambda imm434
RC   lambda 781, lambda 626, lambda 631, lambda 647 from lambda supF
RC   lambda 791 from lambda supF
RC   lambda 678, lambda 679, lambda 709, lambda 696 from lambda, trpE
RC   lambda 760, lambda 742, lambda 705, lambda 762 from lambda, trpE
RA   Murray N.E., Brammar W.J., Murray K.;
RT   "Lambdoid phages that simplify the recovery of in vitro
RT   recombinants";
RL   Mol. Gen. Genet. 150:53-61(1977).
XX
RN   [4]
RP   1-1532
RC   lambda gt10
RA   Kitts P.A.;
RT   "CLONTECH Vectors On Disc version 1.1";
RL   Unpublished (1993).
XX
RN   [5]
RP   1-1532
RC   lambda gt10
RA   Kitts P.A.;
RT   ;
RL   Submitted (07-OCT-1993) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
XX
RN   [6]
RC   lambda imm434 from lambda
RC   lambda imm82 from lambda
RC   lambda MN761 or lambda imm21 from lambda
RA   Kaiser A.D., Jacob F.;
RT   "Recombination between related temperate bacteriophage and the
RT   genetic control of immunity and prophage localisation";
RL   Virology 4:509-521(1957).
XX
RN   [7]
RC   lambda lac from lambda
RC   lambda plac5 or lambda cI857 lacZ from lambda
RA   Ippen K., Shapiro J.A., Beckwith J.R.;
RT   "Transposition of the lac region to the gal region of the
RT   Escherichia coli chromosome: isolation of lambda lac transducing
RT   bacteriophages";
RL   J. Bacteriol. 108:5-9(1971).
XX
RN   [8]
RC   lambda cysB from lambda 569
RC   lambda hisCBHA from lambda
RC   lambda hisG from lambda 540
RC   lambda hsdMS from lambda 569
RC   lambda lig from lambda 569
RC   lambda supD from lambda 569
RC   lambda supE from lambda 569
RC   lambda supF from lambda 540
RC   lambda supF from lambda 569
RC   lambda thyA from lambda 540
RC   lambda tnaA from lambda 540
RC   lambda trpA from lambda 540
RC   lambda trpC from lambda 540
RC   lambda trpE from lambda 540
RA   Borck K., Beggs J.D., Brammar W.J., Hopkins A.S., Murray N.E.;
RT   "The construction in vitro of transducing derivatives ofphage lambda";
RL   Mol. Gen. Genet. 146:199-207(1976).
XX
CC   Left EcoRI arm = 32710 bp. Right EcoRI arm = 10630 bp.
CC   Medium is 1592 SM buffer.
CC   This sequence has been compiled from GenBank accession numbers
CC   Y00118 & J02460; this vector has not been completely sequenced. If
CC   you suspect there is an error in this sequence, please contact
CC   CLONTECH's Technical Service Department at (415) 424-8222 or (800)
CC   662-2566, extension 3 or E-mail CLONTECH@BIOTECHNET.COM.
CC   NCBI gi: 413813
CC   NM (lambda gt10)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (Boehringer)(BRL)(CLONTECH)(Invitrogen)(Promega)(Sigma)
CC   SP (Stratagene)(USB)(Worthington)(ATCC)
CC   HO (E.coli C600)(E.coli C600hflA)(E.coli LE392)
CC   CP ()
CC   FN (amplification)(screening)(cloning cDNA up to 7600 bp)
CC   SE (plaques clear/turbid/no)
CC   PA (lambda)
CC   BR (lambda gt11)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   delete b527, [b2 5986bp 21737..27723]
FT                   2. phage 434 2662bp 35583..38245, immunity region
FT                   -> lambda gt10 43340bp [lambda b527 imm434]"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda A"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda J"
FT   misc_feature    0..0
FT                   /note="deletion b527"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    29490..29490
FT                   /note="SIT unique XhoI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda imm434"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI repressor gene"
FT   misc_binding    32710..32710
FT                   /note="SIT unique EcoRI, in imm434"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cos)"
XX
SQ   Sequence 1532 BP; 486 A; 338 C; 340 G; 368 T; 0 other;
     aagcttcaca ccacgaacca gctctaacca tgctaatcaa tggatatttc cctttgggct
     caacgtgccc aacaaatcta acattcgaat cagaggtgcc attgagcagc cagtcaacac
     ttacgccaag agctgacgca agttctggta aaaagcgtgg tcgcttagtt ttaccgtttt
     cgagctgctc tatagactgc tgggtagtcc ccaccttttg agcaagttca gcctggttaa
     gtccaagctg aattcttttg ctttttaccc tggaagaaat actcataagc cacctctgtt
     atttaccccc aatcttcaca agaaaaactg tatttgacaa acaagataca ttgtatgaaa
     atacaagaaa gtttgttgat ggaggcgata tgcaaactct ttctgaacgc ctcaagaaga
     ggcgaattgc gttaaaaatg acgcaaaccg aactggcaac caaagccggt gttaaacagc
     aatcaattca actgattgaa gctggagtaa ccaagcgacc gcgcttcttg tttgagattg
     ctatggcgct taactgtgat ccggtttggt tacagtacgg aactaaacgc ggtaaagccg
     cttaagacat tcccgctctt acacattcca gccctgaaaa agggcatcaa attaaaccac
     acctatggtg tatgcattta tttgcataca ttcaatcaat tgttatctaa ggaaatactt
     acatatggtt cgtgcaaaca aacgcaacga ggctctacga atcgagagtg cgttgcttaa
     caaaatcgca atgcttggaa ctgagaagac agcggaagct gtgggcgttg ataagtcgca
     gatcagcagg tggaagaggg actggattcc aaagttctca atgctgcttg ctgttcttga
     atggggggtc gttgacgacg acatggctcg attggcgcga caagttgctg cgattctcac
     caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     tcattactgg atctatcaac aggagtcatt atgacaaata cagcaaaaat actcaacttc
     ggcagaggta actttgccgg acaggagcgt aatgtggcag atctcgatga tggttacgcc
     agactatcaa atatgctgct tgaggcttat tcgggcgcag atctgaccaa gcgacagttt
     aaagtgctgc ttgccattct gcgtaaaacc tatgggtgga ataaaccaat ggacagaatc
     accgattctc aacttagcga gattacaaag ttacctgtca aacggtgcaa tgaagccaag
     ttagaactcg tcagaatgaa tattatcaag cagcaaggcg gcatgtttgg accaaataaa
     aacatctcag aatggtgcat ccctcaaaac gagggaaaat cccctaaaac gagggataaa
     acatccctca aattggggga ttgctatccc tcaaaacagg gggacacaaa agacactatt
     acaaaagaaa aaagaaaaga ttattcgtca ga
//