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ID   LAMGT102   preliminary; circular DNA; SYN; 43340 BP.
AC   ATCC77364;
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phage vector lambda gt102 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pRSH from lambda gt10 & pBluescript KS-
RC   plasmid from pRSH & pG5SVK, kan gene
RC   lambda gt101, lambda gt102, lambda gt103 from plasmid & lambda gt10
RA   Lee J., Weissman S.M.;
RT   "Construction of lambda gt103, a derivative of phage lambda gt10
RT   that has unique EcoRI, NotI, SacI and SpeI sites and retains
RT   positive selection for recombinants";
RL   Gene 120:85-88(1992).
RN   [2]
RC   pG5SV from pGEM-5Zf+ & linker & pHAV1, SV40
RC   pG5SVK from pG5SV & linker & pUC4-KIXX, kan gene
RA   Arenstorf H.P., Kandpal R.P., Baskaran N., Parimoo S., Tanaka Y.,
RA   Kitajima S., Yasukochi Y., Weissman S.M.;
RT   "Construction and characterization of a NotI-BsuE linking library
RT   from the human X chromosome";
RL   Genomics 11:115-123(1991).
RN   [3]
RC   pHAV1 from SV40
RA   Vasavada H.A., Ganguly S., Settleman J., DiMaio D., Weissman S.M.;
RT   "A model system for the rescue of cDNA encoding transacting
RT   transcription activator by contingent replication assay";
RL   Indian J. Biochem. Biophys. 25:488-494(1988).
CC   Deposited by: Jinseon Lee, Sherman M. Weissman
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII-->23.0, 9.6, 6.6; BamHI--19.4, 12.0, 6.2, 6.0, 1.5;
CC   PstI--19.4, 6.0, 3.2, 2.7, 2.1, 1.8, 1.25, 1.2, 1.1, 0.95, 0.9, 0.8,
CC   0.6, 0.5, 0.4, 0.3, 0.2, 0.2; EcoRI--33.5, 10.5. (ATCC staff)
CC   Cloning vector permitting positive selection of inserts and useful for
CC   construction of jumping clones and libraries.  Does not
CC   cross-hybridize to sequences found in common plasmid vectors. [1]
CC   DNA inserted into the cloning sites interrupts the imm434 cI
CC   repressor, and thus plaque formation of nonrecombinants is repressed
CC   on high-frequency lysogeny mutant strains such as C600hflA or BNN102.
CC   [1]
CC   The genotype appears to be more stable in the cI434 region than
CC   lambdagt10. (personal communication)
CC   May not be distributed for commercial use. (personal communication)
CC   Differs from lambdagt101 (ATCC 77363) and lambdagt103 (ATCC 77365)
CC   only in the cI434 region. (personal communication)
CC   Medium is 1592 SM buffer.
CC   NM (lambda gt102)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   HO (E.coli)(E.coli C600)(E.coli BNN102)(bacteria-free lysate)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pGEM-5Zf+ 3003bp
FT                   2. linker
FT                   3. pHAV1, SV40
FT                   -> pG5SV 3500bp
FT                   1. pG5SV 3500bp
FT                   2. linker
FT                   3. pUC4-KIXX 1500bp, kan gene
FT                   -> pG5SVK 5000bp
FT                   1. lambda gt10 EcoRI-HindIII 249bp 32710..?, imm434/cI
FT                   2. pBluescript KS+ remove EcoRI-HindIII 12bp 708..720,
FT                   \ MCS/2952bp
FT                   -> pRH 3201bp
FT                   1. pRH EcoRI-HindIII 249bp 32710..?, imm434/cI
FT                   2. pBluescript KS+ remove EcoRI-HindIII 12bp 708..720,
FT                   \ MCS/2952bp
FT                   -> pRNH 3201bp
FT                   1. pRNH small EcoRI-HindIII 249bp 32710..?, imm434/cI
FT                   2. pUC18 remove HindIII-EcoRI 51bp 400..451,
FT                   \ MCS/2635bp
FT                   -> pRSH 3183bp
FT                   1. pRSH NotI 3183bp 671..671
FT                   2. pG5SVK NotI-NotI 1600bp, kan gene
FT                   -> plasmid 4800bp
FT                   1. plasmid EcoRI-HindIII 2000bp, kan gene
FT                   2. lambda gt10 HindIII-XhoI 2960bp ?..29490
FT                   -> fragment 5000bp
FT                   1. fragment EcoRI-XhoI 5000bp
FT                   2. lambda remove small EcoRI-XhoI 1751bp 31748..33499
FT                   -> lambda gt103 43340bp [unique EcoRI-NotI-SacI-SpeI]
FT                   1. lambda gt103 43340bp
FT                   -> lambda gt102 43340bp"
FT   misc_binding    0..0
FT                   /note="MCS unique NotI-SacI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI repressor gene"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda imm434 gene"
SQ   Sequence 480 BP; 122 A; 130 C; 114 G; 114 T; 0 other;
     ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     tcacacagga aacagctatg accatgatta cgccaagctc ggaattaacc ctcactaaag
     ggaacaaaag ctgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttcacac
     cacgaaccag ctctaaccat gctaatcaat ggatatttcc ctttgggctc aacgtgccca
     acaaatctaa cattcgaatc agaggtgcca ttgagcagcc agtcaacact tacgccaaga
     gctgacgcaa gttctggtaa aaagcgtggt cgcttagttt taccgttttc gagctgctct
     atagactgct gggtagtccc caccttttga gcaagttcag cctggttaag tccaagctga
     attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca