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ID   LAMGT11    preliminary; circular DNA; SYN; 43700 BP.
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AC   IG0304; X16973; X04125; ATCC37194;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda gt11 - incomplete, SacI-KpnI + lac/junct.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [lambda gt7-lac5 from lambda gt7 & lac gene]
RC   phage from lambda gt7-lac5 & lambda 540
RC   lambda gt11 or lambda plac5 lacZ from phage & lambda gt4
RA   Young R.A., Davis R.W.;
RT   "Efficient isolation of genes by using antibody probes";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:1194-1198(1983).
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RN   [2]
RC   from lambda gt11 or lambda plac5 lacZ
RA   Young R.A., Davis R.W.;
RT   "Yeast RNA polymerase II genes: isolation with antibody probes";
RL   Science 222:778-782(1983).
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RN   [3]
RC   from lambda gt11 or lambda plac5 lacZ
RA   Mierendorf R.C., Percy C., Young R.A.;
RT   "Gene isolation by screening lambda gt11 libraries with
RT   antibodies";
RL   Meth. Enzymol. 152:458-469(1987).
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RN   [4]
RP   1-1989
RC   lambda gt11 or lambda plac5 lacZ, SacI-KpnI
RA   Slatko B.E.;
RT   ;
RL   Submitted (20-OCT-1989) by:
RL   Slatko B.E., New England Biolabs, Inc., 32 Tozer Road,
RL   Beverly MA 01915, USA.
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RN   [5]
RP   1-1989
RC   from lambda gt11 or lambda plac5 lacZ, SacI-KpnI
RC   from lambda plac5 or lambda cI857 lacZ
RA   Moran L.S., Maina C.V., Poole C.B., Slatko B.E.;
RT   "Nucleotide sequence of the phage lambda-gt11 SacI-KpnI lacZ region";
RL   Gene 93:163-164(1990).
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RN   [6]
RP   1-210
RC   from lambda gt11 or lambda plac5 lacZ, lac gene/lambda phage junction
RA   Chirala S.S.;
RT   "The nucleotide sequence of the lac operon and phage junction in
RT   lambda gt11";
RL   Nucleic Acids Res. 14:5935-5935(1986).
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CC   Left EcoRI arm = 19600 bp. Right EcoRI arm = 24100 bp.
CC   The cI857 repressor is temperature sensitive and so is inactive
CC   at 42 degrees but active at 32 degrees, allowing selection against
CC   lysogeny.
CC   Medium is 1592 SM buffer.
CC   NCBI gi: 58246 & 58114
CC   NM (lambda gt11)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (Boehringer)(BRL)(CLONTECH)(Invitrogen)(NEB)(Pharmacia)(Promega)
CC   SP (Sigma)(Stratagene)(USB)(Worthington)(ATCC)
CC   HO (E.coli Y1088)(E.coli Y1089)(E.coli Y1089r-)
CC   HO (E.coli Y1090)(E.coli Y1090r-)(E.coli C600)
CC   CP ()
CC   FN (cloning 7000-7200 bp)(cloning cDNA)(expression)
CC   FN (antibody screening)(in vitro packaging)
CC   SE (color blue/white)
CC   PA (lambda)
CC   BR ()
CC   OF ()
CC   OR (E.coli Y1090)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda gt4 [lambda cI857 S100 nin5]
FT                   1. lambda
FT                   -> lambda 540 [lambda deltaB imm21 nin5]
FT                   1. lambda
FT                   -> lambda gt7 [lambda b522 nin5]
FT                   1. lambda gt7
FT                   2. E. coli lac gene
FT                   -> lambda gt7-lac5 [lambda lac5 b522 nin5]
FT                   1. lambda gt7-lac5 left end-HindIII, left arm
FT                   2. lambda 540 HindIII-right end, right arm
FT                   -> phage
FT                   1. lambda gt4, right arm
FT                   recombination
FT                   2. phage, left arm
FT                   -> lambda gt11 43700bp
FT                   \ [lambda lac5 cI857 nin5 Sam100; unique EcoRI]"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosL)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda A"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda J"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   misc_feature    1..6
FT                   /note="SIT SacI"
FT   misc_feature    1069..1074
FT                   /note="SIT EcoRI"
FT   misc_feature    1984..1989
FT                   /note="SIT KpnI"
FT   misc_binding    0..0
FT                   /note="SIT KpnI"
FT   misc_binding    0..0
FT                   /note="SIT KpnI"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_feature    1..109
FT                   /note="bacteriophage lambda gt11 lom region"
FT   promoter        80..83
FT                   /note="PRO E. coli lac; pot. -35 region"
FT   promoter        100..105
FT                   /note="PRO E. coli lac; pot. -10 region"
FT   misc_feature    110..110
FT                   /note="junction phage/lac operon"
FT   RBS             124..129
FT                   /note="RBS; potential"
FT   misc_feature    complement(111..>210)
FT                   /note="lac operon 3' end"
FT   CDS             135..>210
FT                   /note="GEN bacteriophage lambda open reading frame;
FT                   NCBI gi: 58115"
FT   misc_binding    19600..19600
FT                   /note="SIT unique EcoRI; in C-terminus of lacZ"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacUV5 gene"
FT   misc_binding    0..0
FT                   /note="SIT SacI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    24820..24820
FT                   /note="SIT unique XbaI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    31500..31500
FT                   /note="SIT unique XhoI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda shndIII2-3"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda cI857"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda nin5"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda S100 amber (Sam100)"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosL)"
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SQ   Sequence 2310 BP; 519 A; 647 C; 580 G; 453 T; 111 other;
     gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct
     ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag
     agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca
     gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg
     ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc
     gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg
     attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg
     gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc
     tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca
     cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta
     tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat
     gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg
     caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc
     cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac
     gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac
     cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa
     accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc
     catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg
     agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg
     ccatgtctgc ccgtatttcg cgtaaggaaa tccattgtac tgccggacca ccgactgtga
     gccactccgg ccatggcgta cgcactgacc tgcttactga tttgtaaaac cggtccggcc
     atcacgctca cataacgtcc acgcaggctc tcatagtgaa acgtatcctc cccggtcatc
     actgtgctgc tctttttcga cgcggcgaac cccagggaag ccatcacccc cacactgtcc
     gtcagctcat aacggtactt cacgttaatc cctttcagat gactcacacc ggtatccccg
     cccgacaacg acggcaatgt acccggtttc acttgaaaat agcccaccgt aaacgtacca
     tgtccacctt ccgcacgggc cggagtgact gtcaccgcaa gtgcggcaaa gacagcaacg
     gcaatacaca cattacgcat cgttcacctc tcactgtttt ataataaaac gcccgttccc
     ggacgaacct ctgtaacaca ctcagaccac gctgatgccc agcgcctgtt tcttaatcac
     cataacctgc acatcgctgg caaacgtata cggcggaata tctgccgaat gccgtgtgga
     cgtaagcgtg aacgtcagga tcacgtttcc ccgacccgct ggcatgtcaa caatacggga
     gaacacctgt accgcctcgt tcgccgcgcc atcataaatc accgcaccgt tcatcagtac
     tttcagataa cacatcgaat acgttgtcct gccgctgaca gtacgcttac ttccgcgaaa
     cgtcagcgga agcaccacta tctggcgatc aaaaggatgg tcatcggtca cggtgacagt
     acgggtaccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     acgttatgtg agcgtgatgg ccggaccggt tttacaaatc agtaagcagg tcagtgcgta
     cgccatggcc ggagtggctc acagtcggtg gtccggcagt acaatggatt tccttacgcg
     aaatacgggc agacatggcc tgcccggtta ttattatttt tgacaccaga ccaactggta
     atggtagcga ccggcgctca gctggaattc
//