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ID   LAMGT11D   preliminary; circular DNA; SYN; 42800 BP.
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AC   IG1013;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda gt11D - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [lambda gt7-lac5 from lambda gt7 & lac gene]
RC   phage from lambda gt7-lac5 & lambda 540
RC   lambda gt11 from phage & lambda gt4
RA   Young R.A., Davis R.W.;
RT   "Efficient isolation of genes by using antibody probes";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:1194-1198(1983).
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CC   Left EcoRI arm = 19600 bp. Right EcoRI arm = 24100 bp.
CC   The cI857 repressor is temperature sensitive and so is inactive
CC   at 42 degrees but active at 32 degrees, allowing selection against
CC   lysogeny.
CC   NM (lambda gt11D)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (Pharmacia)
CC   HO (E.coli Y1088)(E.coli Y1089)(E.coli Y1089r-)
CC   HO (E.coli Y1090)(E.coli Y1090r-)
CC   CP ()
CC   FN (cloning 7000-7200 bp)(cloning cDNA)(expression)
CC   FN (antibody screening)(in vitro packaging)(cloning directional)
CC   SE (color blue/white)
CC   PA (lambda)
CC   BR ()
CC   OF ()
CC   OR (E.coli Y1090)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda gt11, [lambda lac5 cI857 nin5 Sam100]
FT                   2. E. coli kan gene
FT                   -> lambda gt11D 42800bp
FT                   \ [lambda lac5 kan cI857 nin5 Sam100]"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosL)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda A"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda J"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   misc_binding    0..0
FT                   /note="SIT KpnI"
FT   misc_binding    0..0
FT                   /note="SIT KpnI"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI (in C-terminus of lacZ)"
FT   misc_binding    0..0
FT                   /note="SIT unique SfiI (in C-terminus of lacZ)"
FT   misc_binding    0..0
FT                   /note="SIT XhoI (in C-terminus of lacZ)"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacUV5 gene"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   misc_binding    0..0
FT                   /note="SIT SacI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda shndIII2-3"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda cI857"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda nin5"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda S100 amber (Sam100)"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosR)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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