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ID   LAMGTWESBP preliminary; circular DNA; SYN; 1 BP.
AC   ATCC37029;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phage vector lambda gtWES.lambda B' - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   lambda gtWES.lambdaC from lambda gt.lambdaC
RC   lambda gtWES.lambdaB from lambda gtWES.lambdaC & lambda gt.lambdaB
RA   Leder P., Tiemeier D., Enquist L.;
RT   "EK2 derivatives of bacteriophage lambda useful in the cloning of
RT   DNA from higher organisms: the lambdagtWES system";
RL   Science 196:175-177(1977).
CC   A lambda replacement vector.
CC   Medium is 1592 SM buffer.
CC   NM (lambda gtWES.lambda B')
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   HO (bacteria-free lysate)(E.coli DP50 supF)(E.coli LE392)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   duplicate a200 in left arm, to make longer
FT                   substitute b189 for b2, remove EcoRI 1/EcoRI 2
FT                   substitute bio69 for int, remove EcoRI 3
FT                   -> lambda a200 b189 bio69 cI857 nin5, cI857/nin5
FT                   1. lambda a200 b189 bio69 cI857 nin5, cI857/nin5
FT                   EcoRI, EcoRI 4/lambda 39169..39169
FT                   \ lambda EcoRI = 21227 26105 31748 39169 44973
FT                   mutagenesis
FT                   EcoRI, EcoRI 5/lambda 44973..44973
FT                   mutagenesis
FT                   recombination, between beta and N genes
FT                   2. lambda N-, left arm/B fragment/C fragment
FT                   -> lambda gt.lambdaBC
FT                   1. lambda gt.lambdaBC
FT                   remove EcoRI-EcoRI 5400bp, C fragment
FT                   \ lambda 5643bp 26105..31748
FT                   -> lambda gt.lambdaB [left arm/B fragment/right arm]
FT                   1. lambda gt.lambdaBC EcoRI-EcoRI
FT                   remove EcoRI-EcoRI 4690bp, B fragment
FT                   \ lambda 4878bp 21227..26105
FT                   -> lambda gt.lambdaC [left arm/C fragment/right arm]
FT                   1. lambda gt.lambdaC
FT                   mutation Wam403
FT                   mutation Eam100
FT                   mutation Sam100
FT                   -> lambda gtWES.lambdaC
FT                   1. lambda gtWES.lambdaC remove EcoRI-EcoRI, EcoRI C
FT                   2. lambda gt.lambdaB EcoRI-EcoRI, EcoRI B fragment
FT                   -> lambda gtWES.lambdaB'"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;