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ID   LAMJK2     preliminary; circular DNA; SYN; 56 BP.
AC   M28734;
DT   13-APR-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phage vector lambda JK2 - incomplete, promoter.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-56
RC   pUK270 from pUK230
RC   pKS11Xa from pUK270
RC   pJK2, pJK4 from pACYC184 & pKO-2
RC   lambda JK2 from lambda IP1 & pJK2
RC   lambda JK4 from lambda IP1 & pJK4
RC   poT from lambda, ori
RC   pJK2 from lambda JK2 & poT, lambda ori
RC   pJK4 from lambda JK4 & poT, lambda ori
RA   Sieg K., Kun J., Pohl I., Scherf A., Muller-Hill B.;
RT   "A versatile phage lambda expression vector system for cloning in
RT   Escherichia coli";
RL   Gene 75:261-270(1989).
RN   [2]
RC   pUK250 from pUK230
RC   pKO-1 from pUK250 & pMK38-64
RC   pKO2-30 from pKO-1 & linker & pUK250
RC   pKO2-34, pKO2-35, pKO2-36, pKO2-41 from pKO2-30
RC   pKO50 from pKO2-34 & pKO2-41
RC   pKO60 from pKO2-35 & pKO2-41
RC   pKO80 from pKO2-36 & pKO2-41
RC   pKO54 from pKO50
RC   pKO88 from pKO80
RA   Besse M., Von Wilcken-Bergmann B., Muller-Hill B.;
RT   "Synthetic lac operator mediates repression through lac repressor
RT   when introduced upstream and downstream from lac promoter";
RL   EMBO J. 5:1377-1381(1986).
RN   [3]
RC   [lambda NM1151 from lambda]
RC   lambda IP1 from lambda NM1151
RA   Murray N.E.;
RT   "Phage lambda and molecular cloning";
RL   Lambda II 0:395-432(1983).
RL   Hendrix R.W., Roberts J.W., Stahl F.W., Weisberg R.A.,
RL   Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
RN   [4]
RC   pMK38-64 from lacZ gene 3' & amp gene 5'
RA   Von Wilcken-Bergmann B., Koenen M., Grieser H.W., Muller-Hill B.;
RT   ;
RL   EMBO J. 2:1271-1274(1983).
CC   NCBI gi: 208778
CC   NM (lambda JK2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pACYC184 AsnI-BalI 1534bp 1408..2942,
FT                   \ tet/XbaI in amp gene
FT                   2. pKO-2 XhoI 3755bp 33..33, 5' to lacZpo
FT                   XbaI linker 6bp tctaga
FT                   XbaI
FT                   -> plasmid 5289bp
FT                   1. plasmid EcoRI 5289bp 2..2, 3' lacZ/EcoRIcodon 1004
FT                   -> pJK2 6800bp [XhoI 5' to lacZ stop codons]
FT                   1. lambda
FT                   -> lambda NM1151
FT                   1. lambda NM1151 remove HindIII-EcoRI 2974bp,
FT                   \ lambda 23131..26105/lambda XbaI = 24509
FT                   XbaI linker 6bp tctaga
FT                   -> lambda IP1 [unique XbaI]
FT                   1. lambda IP1 XbaI, lambda 23131
FT                   2. pJK2 large XbaI-XbaI 6800bp 39..6800..33, amp/lacZ
FT                   -> lambda JK2"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT E. coli tet gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lacZpo"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-BglII-SpeI-PstI-EcoRI-BamHI-SmaI"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ gene"
SQ   Sequence 56 BP; 16 A; 15 C; 12 G; 13 T; 0 other;
     acgccaagct tacagatctt actagtctgc aggaattcag gatcccgggc aattca