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ID   LAMJK4     preliminary; circular DNA; SYN; 81 BP.
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AC   M28735;
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DT   13-APR-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda JK4 - incomplete, promoter.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-81
RC   pUK270 from pUK230
RC   pKS11Xa from pUK270
RC   pJK2, pJK4 from pACYC184 & pKO-2
RC   lambda JK2 from lambda IP1 & pJK2
RC   lambda JK4 from lambda IP1 & pJK4
RC   poT from lambda, ori
RC   pJK2 from lambda JK2 & poT, lambda ori
RC   pJK4 from lambda JK4 & poT, lambda ori
RA   Sieg K., Kun J., Pohl I., Scherf A., Muller-Hill B.;
RT   "A versatile phage lambda expression vector system for cloning in
RT   Escherichia coli";
RL   Gene 75:261-270(1989).
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CC   NCBI gi: 208779
CC   NM (lambda JK4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pACYC184 AsnI-BalI 1534bp 1408..2942,
FT                   \ tet/XbaI in amp gene
FT                   2. pKO-2 XhoI 3755bp 33..33, 5' to lacZpo
FT                   XbaI linker 6bp tctaga
FT                   XbaI
FT                   -> plasmid 5289bp
FT                   1. plasmid EcoRI 5289bp 2..2, 3' lacZ/EcoRIcodon 1004
FT                   -> pJK4 6800bp [XhoI 5' to lacZ stop codons]
FT                   1. lambda
FT                   -> lambda NM1151
FT                   1. lambda NM1151 remove HindIII-EcoRI 2974bp,
FT                   \ lambda 23131..26105/lambda XbaI = 24509
FT                   XbaI linker 6bp tctaga
FT                   -> lambda IP1 [unique XbaI]
FT                   1. lambda IP1 XbaI, lambda 23131
FT                   2. pJK4 large XbaI-XbaI 6800bp 39..6800..33, amp/lacZ
FT                   -> lambda JK4"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT E. coli tet gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lacZpo"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-BglII-SpeI-PstI-EcoRI-BamHI-SmaI"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ gene"
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SQ   Sequence 81 BP; 27 A; 20 C; 16 G; 18 T; 0 other;
     caaaaactcg aggtaccaag cttacagatc ttactagtct gcaggaattc aggatccccg
     ggcttccaaa tcgagtaata a
//