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ID   LAMMAX1    preliminary; circular DNA; SYN; 43900 BP.
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AC   IG1016;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda MAX1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   lambda MAX1 from lambda
RA   ;
RT   ;
RL   Unpublished (1992).
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CC   NM (lambda MAX1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (CLONTECH)
CC   HO (E.coli)(yeast)
CC   CP ()
CC   FN (cloning cDNA)
CC   SE ()
CC   PA (lambda)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda, cos
FT                   2. pBR322, ori/amp gene
FT                   3. yeast, CEN4
FT                   4. yeast, ARS1
FT                   5. yeast, URA3 gene
FT                   6. yeast, GAL1-GAL10 promoter
FT                   7. T7, promoter
FT                   8. E.coli, lacZ gene
FT                   9. f1, ori
FT                   -> lambda MAX1 43900bp"
FT   misc_binding    19100..19100
FT                   /note="SIT NoeI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    0..0
FT                   /note="yeast centromere CEN4 (for chromosome
FT                   segregation and stability)"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   misc_binding    22900..22900
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene (uracil)"
FT   misc_binding    24000..24000
FT                   /note="SIT EcoRI"
FT   promoter        complement(0..0)
FT                   /note="PRO yeast GAL10 gene"
FT   promoter        0..0
FT                   /note="PRO yeast GAL1 gene"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-XbaI-SalI-XhoI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage f1"
FT   misc_binding    0..0
FT                   /note="SIT NoeI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
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