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ID   LAMNCI857R preliminary; circular DNA; SYN; 1 BP.
AC   ATCC40120;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phage vector lambda N- cI857 r32 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   lambda N- cI857 r32 or lambda Nam7 Nam53 cI857 r32 from lambda papa
RA   ;
RT   ;
RL   Unpublished (1988).
RL   U.S. Patent No. 4,774,182 dated Sept. 27, 1988.
CC   This was isolated as spontaneous mutant of lambda papa prophage. It
CC   contains an IS2 terminator between the tR1 site and the O gene.
CC   This replicates in sup hosts (clear plaques at 42C; turbid plaques at
CC   30C). It does not grow on sup0 hosts and does not kill a sup0 host
CC   when lysogenized and induced at 42C. (personal communication)
CC   This is bacteriophage lambda with mutant O and P genes regulated by
CC   the N product (functional only when N is supplied). It produces
CC   immunity to lambda.
CC   Genotype:Nam7 Nam53 cI857 r32 IS2
CC   Medium is 1592 SM buffer.
CC   NM (lambda N- cI857 r32)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   HO (bacteria-free lysate)(E.coli)(E.coli C600)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda papa
FT                   1. lambda papa, cos
FT                   mutation
FT                   -> lambda N- cI857 r32
FT                   [lambda Nam7 Nam53 cI857 r32 IS2]
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PL"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PR"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;