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ID   LAMNMT     preliminary; circular DNA; SYN; 38000 BP.
AC   ATCC37367;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli phage vector lambda NMT - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pcD-X from pcDV1 & X gene & pL1
RC   pcD*neo from pSV2-neo & pcD-X
RC   lambda NMT from pcD*neo & lambda 762
RA   Okayama H., Berg P.;
RT   "Bacteriophage lambda vector for transducing a cDNA clone library
RT   into mammalian cells";
RL   Mol. Cell. Biol. 5:1136-1142(1985).
CC   Vector for efficient transduction of mammalian cells with a cDNA
CC   library constructed with the pcD (Okayama and Berg) expression vector.
CC   The order of the major features in this bacteriophage is:
CC   lambda762/deltab - HindIII - 0.5 kb SV40 fragment, map units 0.86-0.95
CC   - reddelta - SalI - SV40 ori - SV40 early promoter - SV40 late 19S
CC   and 16S splice junctions - neo (Tn5) - SV40 late poly(A)
CC   - pMB1 ori - ampR - lambda762/KH54/lambda ori/nin5/QSR.
CC   Cloning capacity:12 kb, or 9 kb of cDNA insert and 3 kb of pcD vector.
CC   Medium is 1592 SM buffer.
CC   NM (lambda NMT)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   HO (bacteria-free lysate)(vertebrate cells)(E.coli)
CC   HO (E.coli JC8679 [ATCC 47001 for library amplification])
CC   HO (E.coli C600 [ATCC 23724 for vector amplification])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda 762 [lambda deltab deltared KH54 nin5]
FT                   1. pcDV1 3088bp
FT                   2. X gene
FT                   3. pL1 2600bp
FT                   -> pcD-X 5700bp
FT                   1. pSV2-neo BamHI-BglII 2021bp 752..2773, Tn5 neo gene
FT                   2. pcD-X BamHI 5700bp 2955..2955
FT                   -> pcD*neo 7700bp
FT                   1. lambda 762 HindIII-HindIII 7000bp, supF
FT                   2. SV40 HindIII-HindIII 447bp 1047..1494, 0.86 to 0.95
FT                   -> phage 7500bp
FT                   1. pcD*neo HindIII 7700bp 2536..2536
FT                   Klenow
FT                   Tth111I 7700bp 367..367,
FT                   \ 150bp 3' to pBR322/SV40 polyA junction
FT                   SalI linker 6bp gtcgac
FT                   SalI-SalI 1500bp 2504..?, SV40 prom/splice/neo/polyA
FT                   2. phage XhoI 7500bp
FT                   -> lambda NMT 38000bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-SalI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT mammal G418 resistance gene (G418)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;