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ID   LAMPLACMU1 preliminary; circular DNA; SYN; 1 BP.
AC   ATCC37287;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phage vector lambda plac Mu1 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   lambda pMu507.3 from lambda pMu507
RC   lambda placMu1 from lambda & lambda pMu507
RC   lambda placMu3 from lambda placMu1 & lambda pMu507.3, phage Mu
RC   lambda pmal1043, lambda pmal1044, lambda pmal1045 from lambda placMu1
RC   lambda pmal1046 from lambda placMu1
RC   pGE142 from pBR327
RC   pGE172 from pGE142 & lambda placMu3
RC   pGE226 from pBR327 & pTM2, recA
RA   Bremer E., Silhavy T.J., Weisemann J.M., Weinstock G.M.;
RT   "Lambda placMu: a transposable derivative of bacteriophage lambda
RT   for creating lacZ protein fusions in a single step";
RL   J. Bacteriol. 158:1084-1093(1984).
RN   [2]
RC   pTM2 from ColE1 & Tn3 & recA
RA   Ogawa T., Wabiko H., Tsujimoto T., Horii T., Masukata H., Ogawa H.;
RT   "Characteristics of purified recA protein and the regulation of
RT   its synthesis in vivo";
RL   Cold Spring Harb. Symp. Quant. Biol. 43:909-915(1979).
RN   [3]
RC   from pTM2
RA   Horii T., Ogawa T., Ogawa H.;
RT   "Organization of the recA gene of Escherichia coli";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:313-317(1980).
RN   [4]
RC   lambda pMu507 from lambda & phage Mu, A gene/B gene
RA   Howe M.;
RT   ;
RL   Unpublished (1984).
CC   lambdapMu507 (ATCC 37289) is a helper for lambdaplacMu1.
CC   Genotype: lacZ' lacY Mu attachment sites cI ner(cII)A.
CC   A plaque-forming lambda vector for in vitro construction of lacZ
CC   fusion proteins.
CC   Medium is 1592 SM buffer.
CC   NM (lambda plac Mu1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   HO (bacteria-free lysate)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. phage Mu
FT                   -> phage Mu d11301
FT                   1. phage Mu d11301
FT                   2. lac operon
FT                   -> phage Mu d11301(Ap lac)
FT                   1. lambda
FT                   2. lac operon
FT                   -> lambda p1(209) lacY
FT                   1. lambda p1(209) lacY
FT                   2. Tn901, amp gene/#?
FT                   -> lambda pSG1
FT                   1. lambda pSG1
FT                   recombination
FT                   2. phage Mu d11301(Ap lac)
FT                   -> phage
FT                   1. phage remove phage Mu d11301(Ap lac), Mu att
FT                   2. lambda pMu507
FT                   -> lambda placMu1"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;