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ID   LAMSE4     preliminary; circular DNA; SYN; 46000 BP.
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AC   ATCC37387;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda SE4 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   lambda SE4 from lambda 1059 & pDPT427
RC   pSE103 from pSE101 & lambda, cI857
RC   pSE104 from lambda SE4 & pKB444
RC   pSE106 from pSE104 & pSE101
RC   pSE105 from pSE104 & pSE101
RC   lambda SE6 from lambda SE4 & pSE106
RC   lambda SE5 from lambda SE4 & pSE105
RA   Elledge S.J., Walker G.C.;
RT   "Phasmid vectors for identification of genes by complementation of
RT   Escherichia coli mutants";
RL   J. Bacteriol. 162:777-783(1985).
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RN   [2]
RC   pDPT427 from NR1, cat gene
RC   [pHIQ6 from pHIQ series]
RC   pJJS1002 from pHIQ6 & pPM103 & pJJS300
RC   pBEU27 from pDPT427 & pKN402
RC   pJJS1010 from pBEU27 & pJJS300
RC   pJJS1011 from pJJS1010 & pACYC184
RA   Sninsky J.J., Uhlin B.E., Gustafsson P., Cohen S.N.;
RT   "Construction and characterization of a novel two plasmid system
RT   for accomplishing temperature-regulated, amplified expression of
RT   cloned genes in Escherichia coli";
RL   Gene 16:275-286(1981).
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CC   This vector uses Spi- selection for insertion of DNA into the vector
CC   and has the ability to accept 2 - 19 kb Sau3A1, BamHI, BglII, BclI, or
CC   XhoII fragments. [1]
CC   This vector was constructed by joining the 4.7 kb BamHI-BglII fragment
CC   of pDPT427 (low copy number replicon + spectinomycin resistance) to
CC   the left arm of lambda1059. [1]
CC   With a BamHI digest, the left arm is 24.1 kb, the stuffer fragment
CC   encoding the Spi+ phenotype is 16 kb, and the right arm is 8.6 kb. [2]
CC   This phagemid grows lytically as phage in non-immune hosts or
CC   lysogenically as a phagemid in an immune host. [1]
CC   Plasmid is temperature inducible to produce infectious phage
CC   particles.
CC   A phagemid vector useful for identification of genes by
CC   complementation.
CC   Medium is 1227 LB plus ampicillin. [1]
CC   NM (lambda SE4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (E.coli M5158)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. NR1, ori/spec/strep/cat/#?
FT                   -> pDPT427
FT                   1. lambda 1059 BamHI 45800bp, lambda 27973..27973
FT                   2. pDPT427 BamHI-BglII, cat gene/no ori
FT                   -> lambda SE4 46000bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage NR1"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda Spi+ reporter gene"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli spectinomycin resistance gene
FT                   (spc)"
FT   CDS             0..0
FT                   /note="ANT E. coli streptomycin resistance gene (str)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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