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ID   LAMSE5     preliminary; circular DNA; SYN; 46000 BP.
AC   ATCC37388;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phage vector lambda SE5 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   lambda SE4 from lambda 1059 & pDPT427
RC   pSE103 from pSE101 & lambda, cI857
RC   pSE104 from lambda SE4 & pKB444
RC   pSE106 from pSE104 & pSE101
RC   pSE105 from pSE104 & pSE101
RC   lambda SE6 from lambda SE4 & pSE106
RC   lambda SE5 from lambda SE4 & pSE105
RA   Elledge S.J., Walker G.C.;
RT   "Phasmid vectors for identification of genes by complementation of
RT   Escherichia coli mutants";
RL   J. Bacteriol. 162:777-783(1985).
CC   Plasmid is temperature inducible to produce infectious phage
CC   particles.
CC   A phagemid vector that accepts inserts of 6-24 kb.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (lambda SE5)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   HO (E.coli M5158)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 PvuII 4361bp 2067..2067
FT                   KpnI linker 6bp ggtacc
FT                   -> pKB444 4365bp
FT                   1. lambda SE4 EcoRI-KpnI 11000bp, spec gene
FT                   2. pKB444 EcoRI-KpnI 2293bp 2067..4360, amp gene
FT                   -> pSE104 13300bp
FT                   1. pSE104 large HindIII-SalI, amp/spec gene/no ori
FT                   \ pBR322 30..-..652/11800bp
FT                   2. pSE101 HindIII-SalI, kan gene
FT                   -> pSE105 13000bp
FT                   1. lambda SE4 HindIII-BamHI, spec gene
FT                   double recombination
FT                   2. pSE105 HindIII-BamHI 1200bp, kan gene
FT                   -> lambda SE5 46000bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda Spi+ reporter gene"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli streptomycin resistance gene (str)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;