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ID   LAMSE6     preliminary; circular DNA; SYN; 46000 BP.
AC   ATCC37389;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phage vector lambda SE6 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   lambda SE4 from lambda 1059 & pDPT427
RC   pSE103 from pSE101 & lambda, cI857
RC   pSE104 from lambda SE4 & pKB444
RC   pSE106 from pSE104 & pSE101
RC   pSE105 from pSE104 & pSE101
RC   lambda SE6 from lambda SE4 & pSE106
RC   lambda SE5 from lambda SE4 & pSE105
RA   Elledge S.J., Walker G.C.;
RT   "Phasmid vectors for identification of genes by complementation of
RT   Escherichia coli mutants";
RL   J. Bacteriol. 162:777-783(1985).
CC   Plasmid is temperature inducible to produce infectious phage
CC   particles.
CC   Designed to identify genes by complementation of E.coli host mutations
CC   in hosts producing lambda cI protein.  Accepts inserts of 6-24 kb.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (lambda SE6)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   HO (E.coli M5158)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 PvuII 4361bp 2067..2067
FT                   KpnI linker 6bp ggtacc
FT                   -> pKB444 4365bp
FT                   1. lambda SE4 EcoRI-KpnI 11000bp, spec gene
FT                   2. pKB444 EcoRI-KpnI 2293bp 2067..4360, amp gene
FT                   -> pSE104 13300bp
FT                   1. pSE104 large HindIII-BamHI, amp gene/no spec gene
FT                   \ pBR322 30..-..652/11800bp
FT                   2. pSE101 HindIII-BamHI 1200bp, kan gene
FT                   -> pSE106 13000bp
FT                   1. lambda SE4 HindIII-BamHI, spec gene
FT                   double recombination
FT                   2. pSE106 HindIII-BamHI 1200bp, kan gene
FT                   -> lambda SE6 46000bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage NR1"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda Spi+ reporter gene"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;