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ID   LAMSHLX2   preliminary; circular DNA; SYN; 115 BP.
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AC   M37057;
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DT   15-AUG-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda SHLX2 - incomplete, promoter.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-115
RC   pGLX2 from pZf18R & oligo
RC   lambda GLX from lambda gt10 & pGLX2, loxP
RC   pGLX1 from lambda GLX
RC   [lambda NM1150 from lambda]
RC   lambda PH1 from lambda NM1150 & lambda JP3
RC   lambda PH2 from lambda PH1 & pGLX2
RC   lambda PH3 from lambda PH2 & pC4beta-gal
RC   lambda LOX from lambda PH3
RC   pMP1+ from pGEM-3Zf+ & oligo
RC   pMP1- from pGEM-3Zf- & oligo
RC   pMP2+ from pMP1+ & pET-3xa
RC   pMP2- from pMP1- & pET-3xa
RC   pMP3+ from pMP2+ & oligo
RC   pMP3- from pMP2- & oligo
RC   lambda EXLX+ from pMP3+ & lambda LOX
RC   lambda EXLX- from pMP3- & lambda LOX
RC   pDD1, pDD2 from pCM1 & oligos
RC   lambda SHLX1 from pDD1 & lambda LOX
RC   lambda SHLX2 from pDD2 & lambda LOX
RA   Palazzolo M.J., Hamilton B.A., Ding D., Martin C.H., Mead D.A.,
RA   Mierendorf R.C., Raghavan K.V., Meyerowitz E.M., Lipshitz H.D.;
RT   "Phage lambda cDNA cloning vectors for subtractive hybridization,
RT   fusion-protein synthesis and Cre-loxP automatic plasmid subcloning";
RL   Gene 88:25-36(1990).
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CC   NCBI gi: 208753
CC   NM (lambda SHLX2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (lambda SHLX)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM3Zf+ large PvuII-PvuII 2820bp 270..3090
FT                   2. pATH1 NruI-AvaI 944bp 476..1419, trpE gene
FT                   \ may be HaeII-SacI 470..1416/may be SmaI 1421
FT                   -> pCM1 3764bp
FT                   1. pCM1 remove SacI-HindIII 41bp 16..57,
FT                   \ MCS/pGEM3Zf+/3723bp
FT                   2. oligo SacI-EcoRI-XbaI-ApaI-HindIII 33bp
FT                   -> pDD2 3745bp
FT                   1. lambda
FT                   -> lambda NM1150
FT                   1. lambda NM1150 ApaI, lambda 10091..10091
FT                   fill in
FT                   make ApaI in right arm
FT                   -> m lambda JP3
FT                   1. lambda NM1150 ApaI, lambda 10091..10091
FT                   fill in
FT                   make ApaI in right arm
FT                   -> m lambda JP3
FT                   1. lambda NM1150 SnaBI-right end 36311bp,
FT                   \ lambda 12191..48502
FT                   2. m lambda JP3 left end-SnaBI 12191bp,
FT                   \ lambda 0..12191
FT                   -> lambda PH1 48502bp
FT                   1. pZF18R SmaI 2900bp, MCS
FT                   \ pTZ18R SmaI 2861bp 200..200
FT                   2. oligo loxP-SmaI-loxP 74bp [34+6+34]
FT                   -> pGLX2 3000bp
FT                   1. lambda PH1 XhoI 48502bp, lambda 33499..33499
FT                   2. pGLX2 XhoI-XhoI 100bp, loxP sites
FT                   -> lambda PH2 48500bp
FT                   1. pSV2-dhfr 5000bp
FT                   2. Carnegie 4 3630bp
FT                   -> plasmid 8600bp
FT                   1. pMLB1034 6189bp
FT                   linker
FT                   -> pCGE41 6200bp
FT                   1. plasmid 8600bp
FT                   2. pCGE41, lacZ gene/pMLB1034 3000..6000
FT                   -> pC4beta-gal
FT                   1. lambda PH2 remove SacI-XbaI, pGLX2
FT                   2. pC4beta-gal SacI-XbaI 850bp, SV40 sequence
FT                   -> lambda PH3
FT                   1. lambda PH3 EcoRI-HindIII
FT                   Klenow:Klenow
FT                   -> lambda LOX
FT                   1. pDD2 HpaI 3756bp, pATH1 250..250
FT                   2. lambda LOX SacI-XbaI
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   -> lambda SHLX2"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
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SQ   Sequence 115 BP; 28 A; 31 C; 28 G; 28 T; 0 other;
     ggccaagtcg gcctctaata cgactcacta tagggagcta agcttgggcc ctctagacag
     gaattcgagc tcagcttgat gcatattcta tagtgtcacc taaatctgcg gccgc
//