back Return to this vector's summary.
ID   LAMSK      preliminary; circular DNA; SYN; 1 BP.
XX
AC   IG5086;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
XX
DE   E. coli plasmid vector lambda SK - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   lambda SK4 from EMBL3 & oligo
RC   lambda SK6 from lambda 2001 & oligo
RC   lambda SK10 from lambda SK9 & oligo
RC   lambda SK20 from lambda SK10 & oligo
RC   lambda SK21 from lambda SK9 & lambda GEM11
RC   lambda SK22 from lambda SK9 & oligo
RC   lambda SR9-B from lambda SK18 & oligo
RC   lambda SK23 from lambda SK10 & EMBL3 & lambda SR9-B
RC   lambda SK24 from lambda SK22 & EMBL3 & lambda SR9-B
RC   lambda SK25 from lambda SK22 & oligo
RC   lambda SK27 from lambda SK22
RC   lambda SK40 from lambda SK10 & oligo
RC   lambda SK41 from lambda SK10 & oligo
RC   lambda SK16 from lambda SK15 & oligo
RC   lambda SK17 from lambda SK15 & oligo
RC   lambda SK28 from lambda SK17
RA   Zabarovsky E.R., Klein G., Winberg G.;
RT   "Lambda SK diphasmids: phage lambda vectors for genomic, jumping,
RT   linking and cDNA libraries";
RL   Gene 127:1-14(1993).
XX
RN   [2]
RC   placZ from pBR322 & Charon 4A, lacZ gene
RC   phage EL3/4 from EMBL3 & EMBL4 & placZ
RC   lambda SK3 from EMBL3 & phage EL3/4
RC   phage SK-P from EMBL4 & linker
RC   lambda SK5 from lambda SK3 & phage SK-P
RC   lambda SK1, lambda SK2, lambda SK6 from pHC79 & M13mp18
RC   lambda SK18, lambda SK18' from pHC79 & M13mp18
RC   lambda SK2A from lambda SK18
RC   lambda SK2deltaSR from lambda SK2
RC   lambda SK9 from EMBL3 & lambda SK2deltaSR
RC   lambda SK10 from lambda SK9 & oligo
RC   lambda SK11 from lambda gt11 & oligo
RC   lambda SK12 from lambda SK2A & pE3/4
RC   lambda SK15 from lambda SK9 & pUC4K
RC   lambda SK16 from lambda SK15 & oligo
RC   lambda SK17 from lambda SK15 & oligo
RC   lambda SK20 from lambda SK10 & oligo
RC   lambda SK21 from lambda SK9 & pGEM11
RC   lambda SK22 from lambda SK9 & oligo
RC   pSR9 from lambda SK2
RC   lambda SK23 from lambda SK10 & oligo & pSR9
RC   lambda SK24 from lambda SK22 & lambda SK23
RC   M13mc18 from M13mp18 & pHC79
RC   M13mc19 from M13mp19 & pHC79
RA   Zabarovsky E.R., Winberg G.;
RT   "Improved methods and new vectors for creating the clone libraries
RT   of genes";
RL   Sov. Sci. Rev. D. Physiochem. Biol. 11:1-65(1992).
XX
RN   [3]
RC   lambda pMYF15 from piAN7 & lambda L47.1AB
RC   lambda pMYF17 from lambda pMYF15 & lambda gtWES.lambdaC
RC   lambda pMYF131 from lambda pMYF17 & pUC19
RC   lambda pSL5 from lambda pMYF131 & lambda L47.1
RC   lambda pMK2 from lambda pMYF131 & ptl gene
RA   Yankovsky N.K., Fonstein M.Y., Laskina S.Y., Bukanov N.O.,
RA   Yakubovich N.V., Ermakova L.M., Rebentish B.A., Janulaitis A.A.,
RA   Debabov V.G.;
RT   "Phasmids as effective and simple tools for construction and
RT   analysis of gene libraries";
RL   Gene 81:203-210(1989).
XX
RN   [4]
RC   SK18 from lambda
RA   Zabarovsky E.R., Winberg G., Boldog F., Turina O.T., Nurbekov M.K.,
RA   Marcsek Z., Erlandsson R., Grigorieva A.Y., Stanbridge E.J.,
RA   Sumegi J., Kisselev L.L., Klein G.;
RT   "New generation of vectors for cloning and mapping of the human
RT   genome";
RL   Adv. Mol. Gen. 4:281-293(1991).
XX
RN   [5]
RC   [lambda 47.1c+ from lambda]
RC   [lambda L47.1, lambda L47.1AB from lambda]
RC   lambda pMYF11 from pBR322 & lambda 47.1c+
RA   Yankovsky N.K., Fonstein M.Y., Debabov V.G.;
RT   "Monomer circular phasmid DNA molecules can be effectively packaged
RT   in vitro and used as vector DNA molecules";
RL   Biotech 85 Europe 85:709-714(1985).
XX
CC   MCS. oligonucleotide linker.
CC   NM (lambda SK)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (EMBL3)(lambda 2001)(lambda SK3)(lambda SK5)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pHC79 EcoRI-SalI, lambda cos
FT                   Klenow fill in:Klenow fill in
FT                   2. M13mp18 BalI-BglII 1860bp, M13 ori/lacI/lacZ/MCS
FT                   Klenow fill in:Klenow fill in
FT                   -> lambda SK1"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//