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ID   LAMSK17    preliminary; circular DNA; SYN; 51000 BP.
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AC   ATCC86969;
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DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage lambdaSK17 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   placZ from pBR322 & Charon 4A, lacZ gene
RC   phage EL3/4 from EMBL3 & EMBL4 & placZ
RC   lambda SK3 from EMBL3 & phage EL3/4
RC   phage SK-P from EMBL4 & linker
RC   lambda SK5 from lambda SK3 & phage SK-P
RC   lambda SK1, lambda SK2, lambda SK6 from pHC79 & M13mp18
RC   lambda SK18, lambda SK18' from pHC79 & M13mp18
RC   lambda SK2A from lambda SK18
RC   lambda SK2deltaSR from lambda SK2
RC   lambda SK9 from EMBL3 & lambda SK2deltaSR
RC   lambda SK10 from lambda SK9 & oligo
RC   lambda SK11 from lambda gt11 & oligo
RC   lambda SK12 from lambda SK2A & phage EL3/4
RC   lambda SK15 from lambda SK9 & pUC4K
RC   lambda SK16 from lambda SK15 & oligo
RC   lambda SK17 from lambda SK15 & oligo
RC   lambda SK20 from lambda SK10 & oligo
RC   lambda SK21 from lambda SK9 & pGEM-11Zf+
RC   lambda SK22 from lambda SK9 & oligo
RC   pSR9 from lambda SK2
RC   lambda SK23 from lambda SK10 & oligo & pSR9
RC   lambda SK24 from lambda SK22 & lambda SK23
RC   M13mc18 from M13mp18 & pHC79
RC   M13mc19 from M13mp19 & pHC79
RA   Zabarovsky E.R., Winberg G.;
RT   "Improved methods and new vectors for creating the clone libraries
RT   of genes";
RL   Sov. Sci. Rev. D. Physiochem. Biol. 11:1-65(1992).
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RN   [2]
RC   lambda SK4 from EMBL3 & oligo
RC   lambda SK6 from lambda 2001 & oligo
RC   lambda SK10 from lambda SK9 & oligo
RC   lambda SK20 from lambda SK10 & oligo
RC   lambda SK21 from lambda SK9 & lambda GEM11
RC   lambda SK22 from lambda SK9 & oligo
RC   lambda SR9-B from lambda SK18 & oligo
RC   lambda SK23 from lambda SK10 & EMBL3 & lambda SR9-B
RC   lambda SK24 from lambda SK22 & EMBL3 & lambda SR9-B
RC   lambda SK25 from lambda SK22 & oligo
RC   lambda SK27 from lambda SK22
RC   lambda SK40 from lambda SK10 & oligo
RC   lambda SK41 from lambda SK10 & oligo
RC   lambda SK16 from lambda SK15 & oligo
RC   lambda SK17 from lambda SK15 & oligo
RC   lambda SK28 from lambda SK17
RA   Zabarovsky E.R., Klein G., Winberg G.;
RT   "Lambda SK diphasmids: phage lambda vectors for genomic, jumping,
RT   linking and cDNA libraries";
RL   Gene 127:1-14(1993).
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CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--25.0, 13.5 (doublet); EcoRI--25.0, 13.5 (doublet); SalI--21.0,
CC   8.8, 8.2, 5.0, 4.4, 3.6. (ATCC staff)
CC   Expression vector permitting positive selection (Spi) of
CC   recombinants and control of expression using ochre (supB, supC and/or
CC   supG, supV) and amber (supE, supF) suppressors. Useful for conversion
CC   to phagemid.
CC   Permits expression in three reading frames, construction using
CC   partial filling-in, and oriented cloning. The sizes of the major
CC   components are (kb): arms--20.3, 12.7; stuffer fragment--13.7;
CC   phagemid segments--3.9 (longer arm), 0.7. The polylinker sites given
CC   are those associated with the longer arm.  The sites associated with
CC   the other arm are: SalI BamHI EcoRI XhoI NotI NaeI SfiI AvrII NheI
CC   NcoI XbaI. The restriction sites to be used for conversion to phagemid
CC   are: SalI, ClaI.
CC   Cloning capacity: 0.2 - 15.4 kb. The order of the major features in
CC   this plasmid is: arm - ClaI - BspMII - SalI - pMB1 ori - ampR -
CC   M13 ori - SacI/MCS/ SalI --> - stuffer - SalI/MCS/XbaI/lacZ' -
CC   ClaI - SalI - arm. [2]
CC   Deposited by: Zabarovsky E.R., Winberg G.R., Klein G.R.
CC   NM (lambdaSK17)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(expression)(DNA library)
CC   SE (Spi+)
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda 48502bp, ori
FT                   2. M13, ori
FT                   3. pBR322, ori
FT                   -> lambdaSK17 51000bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli phage lambda"
FT   rep_origin      0..0
FT                   /note="ORI E. coli phage M13"
FT   CDS             0..0
FT                   /note="ANT E. coli red+ gene"
FT   CDS             0..0
FT                   /note="ANT E. coli gam+ gene"
FT   misc_binding    0..0
FT                   /note="MCS SacI-KpnI-SmaI-NcoI-NheI-AvrII-SfiI-NaeI-
FT                   NotI-XhoI-EcoRI-BamHI-SalI/SalI-BamHI-EcoRI-XhoI-
FT                   NotI-NaeI-AvrII-NheI-NcoI-XbaI-PvuI-PstI-SphI-HindIII"
FT   promoter        0..0
FT                   /note="PRO E. coli lac"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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