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ID   LAMSK23    preliminary; circular DNA; SYN; 47800 BP.
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AC   ATCC86973;
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DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage lambdaSK23 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   placZ from pBR322 & Charon 4A, lacZ gene
RC   phage EL3/4 from EMBL3 & EMBL4 & placZ
RC   lambda SK3 from EMBL3 & phage EL3/4
RC   phage SK-P from EMBL4 & linker
RC   lambda SK5 from lambda SK3 & phage SK-P
RC   lambda SK1, lambda SK2, lambda SK6 from pHC79 & M13mp18
RC   lambda SK18, lambda SK18' from pHC79 & M13mp18
RC   lambda SK2A from lambda SK18
RC   lambda SK2deltaSR from lambda SK2
RC   lambda SK9 from EMBL3 & lambda SK2deltaSR
RC   lambda SK10 from lambda SK9 & oligo
RC   lambda SK11 from lambda gt11 & oligo
RC   lambda SK12 from lambda SK2A & phage EL3/4
RC   lambda SK15 from lambda SK9 & pUC4K
RC   lambda SK16 from lambda SK15 & oligo
RC   lambda SK17 from lambda SK15 & oligo
RC   lambda SK20 from lambda SK10 & oligo
RC   lambda SK21 from lambda SK9 & pGEM-11Zf+
RC   lambda SK22 from lambda SK9 & oligo
RC   pSR9 from lambda SK2
RC   lambda SK23 from lambda SK10 & oligo & pSR9
RC   lambda SK24 from lambda SK22 & lambda SK23
RC   M13mc18 from M13mp18 & pHC79
RC   M13mc19 from M13mp19 & pHC79
RA   Zabarovsky E.R., Winberg G.;
RT   "Improved methods and new vectors for creating the clone libraries
RT   of genes";
RL   Sov. Sci. Rev. D. Physiochem. Biol. 11:1-65(1992).
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RN   [2]
RC   lambda SK4 from EMBL3 & oligo
RC   lambda SK6 from lambda 2001 & oligo
RC   lambda SK10 from lambda SK9 & oligo
RC   lambda SK20 from lambda SK10 & oligo
RC   lambda SK21 from lambda SK9 & lambda GEM11
RC   lambda SK22 from lambda SK9 & oligo
RC   lambda SR9-B from lambda SK18 & oligo
RC   lambda SK23 from lambda SK10 & EMBL3 & lambda SR9-B
RC   lambda SK24 from lambda SK22 & EMBL3 & lambda SR9-B
RC   lambda SK25 from lambda SK22 & oligo
RC   lambda SK27 from lambda SK22
RC   lambda SK40 from lambda SK10 & oligo
RC   lambda SK41 from lambda SK10 & oligo
RC   lambda SK16 from lambda SK15 & oligo
RC   lambda SK17 from lambda SK15 & oligo
RC   lambda SK28 from lambda SK17
RA   Zabarovsky E.R., Klein G., Winberg G.;
RT   "Lambda SK diphasmids: phage lambda vectors for genomic, jumping,
RT   linking and cDNA libraries";
RL   Gene 127:1-14(1993).
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CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--23.0, 14.0, 11.0; SalI--21.0, 11.0, 9.0, 6.6; XhoI--23.0, 14.0,
CC   11.0. (ATCC staff)
CC   Vector permitting positive selection (Spi) of recombinants. Useful
CC   for generating jumping and linking libraries and conversion to
CC   phagemid (with selection).
CC   Permits construction using partial filling-in.
CC   The sizes of the major components are (kb): arms--20.3, 9.2; stuffer
CC   fragment--13.7; phagemid segments--2.6 (longer arm), 1.9.
CC   The restriction sites to be used for conversion to phagemid are:
CC   SalI ApaI RsrII NheI. Cloning capacity: 18.9 kb.
CC   The order of the major features
CC   in this plasmid is: arm - SalI/NheI - ampR - M13 ori - SacII/MCS/NaeI
CC   - stuffer - NaeI/MCS/XbaI - pMB1 ori - NheI/SalI - arm.
CC   Deposited by: Zabarovsky E.R., Winberg G.R., Klein G.R.
CC   NM (lambdaSK23)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(DNA library)(sequencing)(RNA synthesis in vitro)
CC   SE (Spi+)
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda 48502bp, ori
FT                   2. M13, ori
FT                   3. pBR322, ori
FT                   -> lambdaSK23 47800bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli phage lambda"
FT   rep_origin      0..0
FT                   /note="ORI E. coli phage M13"
FT   CDS             0..0
FT                   /note="ANT E. coli red+ gene"
FT   CDS             0..0
FT                   /note="ANT E. coli gam+ gene"
FT   misc_binding    0..0
FT                   /note="MCS SacI-KpnI-SmaI-BamHI-XhoI-EcoRI-AvrII-
FT                   NaeI/NaeI-AvrII-EcoRI-XhoI-BamHI-XbaI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
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