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ID   LAMSURFZAP preliminary; circular DNA; SYN; 1 BP.
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AC   IG2053; X04125; X16973;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda SurfZAP - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   lambda SurfZAP from lambda
RA   Amberg J.;
RT   ;
RL   Strategies 6:2-4(1993).
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RN   [2]
RC   lambda ImmunoZAP 13 from lambda ImmunoZAP L & Fab oligo
RA   Hogrefe H.H., Mullinax R.L., Lovejoy A.E., Hay B.N., Sorge J.A.;
RT   "A bacteriophage lambda vector for the cloning and expression of
RT   immunoglobulin Fab fragments on teh surface of filamentous phage";
RL   Gene 128:119-126(1993).
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RN   [3]
RC   lambda ImmunoZAP H from lambda ZAP II & IgG heavy chain DNA
RC   lambda ImmunoZAP L from lambda ZAP II & IgG light chain DNA
RC   lambda ImmunoZAP H/L from lambda ImmunoZAP L & lambda ImmunoZAP H
RA   Mullinax R.L., Gross E.A., Amberg J.R., Hay B.N., Hogrefe H.H.,
RA   Kubitz M.M., Greener A., Alting-Mees M., Ardourel D., Short J.M.,
RA   Sorge J.A., Shopes B.;
RT   "Identification of human antibody fragment clones specific for
RT   tetanus toxoid in a bacteriophage lambda immunoexpression system";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:8095-8099(1990).
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RN   [4]
RC   lambda SurfZAP from lambda
RA   Shopes B.;
RT   "Bridging the barriers to antibody discovery by recombinant DNA
RT   techniques";
RL   Third Annual IBC Intl. Conf. Antibody Engineering 0:121-131(1992).
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CC   The cI857 repressor is temperature sensitive and so is inactive
CC   at 42 degrees but active at 32 degrees, allowing selection against
CC   lysogeny.
CC   Contains phagemid Bluescript SK-.
CC   NM (lambda SurfZAP)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (Stratagene)
CC   HO (E.coli XL-1-Blue MRF')
CC   CP ()
CC   FN (cloning up to 10000 bp)(cloning cDNA)(cloning unidirectional)
CC   FN (expression prokaryote)(antibody screening)(excision automatic)
CC   SE (color blue/white)
CC   PA (lambda)
CC   BR (lambda ZAP Express)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda ZAP II
FT                   2. IgG light chain DNA
FT                   -> lambda ImmunoZAP L
FT                   1. human IgG1 gene
FT                   PCR adds SacI/XbaI/stop codons/spacer
FT                   2. M13, III gene
FT                   PCR adds spacer/stop codon/SpeI
FT                   -> product
FT                   1. product SacI-SpeI, human IgG1 gene/M13 III gene
FT                   2. lambda ImmunoZAP L remove small SacI-XbaI, lacZ/I
FT                   -> lambda ImmunoZAP 13
FT                   1. lambda ImmunoZAP 13
FT                   -> lambda SurfZAP"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosL)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda A"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda J"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacUV5"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   misc_feature    0..0
FT                   /note="E. coli pelB leader sequence"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   CDS             complement(0..0)
FT                   /note="GEN bacteriophage lambda protein coding
FT                   sequence"
FT   misc_binding    0..0
FT                   /note="SIT SpeI"
FT   misc_feature    0..0
FT                   /note="GGGGS"
FT   misc_feature    0..0
FT                   /note="gIII"
FT   misc_feature    0..0
FT                   /note="stop codons"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   misc_feature    0..0
FT                   /note="TER helper phage initiator"
FT   misc_feature    0..0
FT                   /note="TER helper phage terminator"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1-"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda shndIII2-3"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda cI857"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda nin5"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda S100 amber (Sam100)"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosL)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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