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ID LAMSURFZAP preliminary; circular DNA; SYN; 1 BP.
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AC IG2053; X04125; X16973;
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DT 03-FEB-1994 (Rel. 8, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phage vector lambda SurfZAP - incomplete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC lambda SurfZAP from lambda
RA Amberg J.;
RT ;
RL Strategies 6:2-4(1993).
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RN [2]
RC lambda ImmunoZAP 13 from lambda ImmunoZAP L & Fab oligo
RA Hogrefe H.H., Mullinax R.L., Lovejoy A.E., Hay B.N., Sorge J.A.;
RT "A bacteriophage lambda vector for the cloning and expression of
RT immunoglobulin Fab fragments on teh surface of filamentous phage";
RL Gene 128:119-126(1993).
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RN [3]
RC lambda ImmunoZAP H from lambda ZAP II & IgG heavy chain DNA
RC lambda ImmunoZAP L from lambda ZAP II & IgG light chain DNA
RC lambda ImmunoZAP H/L from lambda ImmunoZAP L & lambda ImmunoZAP H
RA Mullinax R.L., Gross E.A., Amberg J.R., Hay B.N., Hogrefe H.H.,
RA Kubitz M.M., Greener A., Alting-Mees M., Ardourel D., Short J.M.,
RA Sorge J.A., Shopes B.;
RT "Identification of human antibody fragment clones specific for
RT tetanus toxoid in a bacteriophage lambda immunoexpression system";
RL Proc. Natl. Acad. Sci. U.S.A. 87:8095-8099(1990).
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RN [4]
RC lambda SurfZAP from lambda
RA Shopes B.;
RT "Bridging the barriers to antibody discovery by recombinant DNA
RT techniques";
RL Third Annual IBC Intl. Conf. Antibody Engineering 0:121-131(1992).
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CC The cI857 repressor is temperature sensitive and so is inactive
CC at 42 degrees but active at 32 degrees, allowing selection against
CC lysogeny.
CC Contains phagemid Bluescript SK-.
CC NM (lambda SurfZAP)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)(linear)
CC ST ()
CC TY (phage)
CC SP (Stratagene)
CC HO (E.coli XL-1-Blue MRF')
CC CP ()
CC FN (cloning up to 10000 bp)(cloning cDNA)(cloning unidirectional)
CC FN (expression prokaryote)(antibody screening)(excision automatic)
CC SE (color blue/white)
CC PA (lambda)
CC BR (lambda ZAP Express)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. lambda ZAP II
FT 2. IgG light chain DNA
FT -> lambda ImmunoZAP L
FT 1. human IgG1 gene
FT PCR adds SacI/XbaI/stop codons/spacer
FT 2. M13, III gene
FT PCR adds spacer/stop codon/SpeI
FT -> product
FT 1. product SacI-SpeI, human IgG1 gene/M13 III gene
FT 2. lambda ImmunoZAP L remove small SacI-XbaI, lacZ/I
FT -> lambda ImmunoZAP 13
FT 1. lambda ImmunoZAP 13
FT -> lambda SurfZAP"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cohesive end site
FT (cosL)"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT CDS 0..0
FT /note="GEN bacteriophage lambda A"
FT CDS 0..0
FT /note="GEN bacteriophage lambda J"
FT CDS complement(0..0)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT promoter complement(0..0)
FT /note="PRO E. coli lacUV5"
FT promoter 0..0
FT /note="PRO bacteriophage T3"
FT misc_feature 0..0
FT /note="E. coli pelB leader sequence"
FT misc_binding 0..0
FT /note="SIT NotI"
FT CDS complement(0..0)
FT /note="GEN bacteriophage lambda protein coding
FT sequence"
FT misc_binding 0..0
FT /note="SIT SpeI"
FT misc_feature 0..0
FT /note="GGGGS"
FT misc_feature 0..0
FT /note="gIII"
FT misc_feature 0..0
FT /note="stop codons"
FT promoter complement(0..0)
FT /note="PRO bacteriophage T7"
FT misc_feature 0..0
FT /note="TER helper phage initiator"
FT misc_feature 0..0
FT /note="TER helper phage terminator"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1-"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT misc_binding 0..0
FT /note="SIT XhoI"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT misc_binding 0..0
FT /note="SIT PvuI"
FT CDS 0..0
FT /note="GEN bacteriophage lambda shndIII2-3"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT CDS 0..0
FT /note="GEN bacteriophage lambda cI857"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT CDS 0..0
FT /note="GEN bacteriophage lambda nin5"
FT CDS 0..0
FT /note="GEN bacteriophage lambda S100 amber (Sam100)"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cohesive end site
FT (cosL)"
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SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
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