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ID LAMZAPEXP preliminary; circular DNA; SYN; 1 BP.
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AC IG2041; X04125; X16973;
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DT 03-FEB-1994 (Rel. 8, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phage vector lambda ZAP Express - incomplete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pJF3 from pUC19 & T7 oligo
RC pBS or BlueScribe from pJF3 & T3 oligo
RC pBS+, pBS- from pBS or BlueScribe & pEMBL8
RC pBluescript KS(+) from pBS+ & linker
RC pBluescript KS(-) from pBS- & linker
RC pBST-B from pBS & pEMBL8
RC pBSITO#12 from pBST-B & pEMBL8
RC pPreB from pBSITO#12 & pBluescript SK(-)
RC lambda cI857 Sam7, lambda cI857 Sam100 nin5 from lambda
RC lambda LongC from lambda cI857 Sam7 & pUC19 & lambda cI857 Sam100 nin5
RC lambda LongD from lambda LongC
RC lambda ZAP from lambda L47.1 & pPreB & lambda LongD
RA Short J.M., Fernandez J.M., Sorge J.A., Huse W.D.;
RT "Lambda ZAP: a bacteriophage lambda expression vector with in vivo
RT excision properties";
RL Nucleic Acids Res. 16:7583-7600(1988).
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RN [2]
RC pBK-RSV from pBR322 & RSV
RC pBK-CMV from pBR322 & CMV
RC lambda ZAP Express from lambda & pBK-CMV
RA Alting-Mees M.A.;
RT ;
RL Strategies 5:58-61(1992).
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RN [3]
RC pBK-RSV from pBR322 & RSV
RC pBK-CMV from pBR322 & CMV
RC lambda ZAP Express from lambda & pBK-CMV
RA Alting-Mees M.A., Vaillancourt P., Short J.M.;
RT ;
RL Plasmids A Practical Approach 0:0-0(1993).
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CC The cI857 repressor is temperature sensitive and so is inactive
CC at 42 degrees but active at 32 degrees, allowing selection against
CC lysogeny.
CC Contains phagemid pBK-CMV.
CC NM (lambda ZAP Express)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)(linear)
CC ST ()
CC TY (phage)
CC SP (Stratagene)
CC HO (E.coli XL-1-Blue MRF')
CC CP ()
CC FN (cloning up to 12000 bp)(cloning cDNA)(cloning unidirectional)
CC FN (expression prokaryote)(expression eukaryote)(excision automatic)
CC SE (color blue/white)
CC PA (lambda)
CC BR (lambda ZAP II)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322
FT 2. CMV
FT -> pBK-CMV
FT 1. lambda
FT 2. pBK-CMV
FT -> lambda ZAP Express"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cohesive end site
FT (cosL)"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT CDS 0..0
FT /note="GEN bacteriophage lambda A"
FT CDS 0..0
FT /note="GEN bacteriophage lambda J"
FT CDS 0..0
FT /note="GEN bacteriophage lambda bio"
FT misc_binding 0..0
FT /note="SIT NsiI"
FT promoter complement(0..0)
FT /note="PRO CMV immediate-early"
FT misc_binding 0..0
FT /note="SIT NheI"
FT CDS complement(0..0)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT promoter complement(0..0)
FT /note="PRO E. coli lacUV5 gene"
FT promoter 0..0
FT /note="PRO bacteriophage T3"
FT misc_binding 0..0
FT /note="MCS SacI-EcoRI-KpnI"
FT misc_binding 0..0
FT /note="SIT unique EcoRI (in C-terminus of lacZ)"
FT promoter complement(0..0)
FT /note="PRO bacteriophage T7"
FT misc_binding 0..0
FT /note="SIT SV40 3' splice site"
FT polyA_signal 0..0
FT /note="PLA SV40 polyA site"
FT misc_binding 0..0
FT /note="SIT MluI"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1-"
FT misc_binding 0..0
FT /note="SIT SspI"
FT misc_feature 0..0
FT /note="TER helper phage initiator"
FT misc_feature 0..0
FT /note="TER helper phage terminator"
FT misc_binding 0..0
FT /note="SIT NaeI"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 0..0
FT /note="SIT SspI"
FT misc_binding 0..0
FT /note="SIT MstII"
FT rep_origin 0..0
FT /note="ORI SV40"
FT CDS complement(0..0)
FT /note="ANT E. coli neomycin phosphotransferase gene
FT (NPT); neomycin resistance gene (neo)"
FT CDS complement(0..0)
FT /note="ANT E. coli kanamycin resistance gene (kan)"
FT polyA_signal 0..0
FT /note="PLA mammal thymidine kinase polyA site"
FT misc_binding 0..0
FT /note="SIT MstII"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT misc_binding 0..0
FT /note="SIT XhoI"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT misc_binding 0..0
FT /note="SIT PvuI"
FT CDS 0..0
FT /note="GEN bacteriophage lambda shndIII2-3"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT CDS 0..0
FT /note="GEN bacteriophage lambda cI857"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT CDS 0..0
FT /note="GEN bacteriophage lambda nin5"
FT CDS 0..0
FT /note="GEN bacteriophage lambda S100 amber (Sam100)"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cohesive end site
FT (cosL)"
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SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
n
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