back Return to this vector's summary.
ID   LAMZAPEXP  preliminary; circular DNA; SYN; 1 BP.
XX
AC   IG2041; X04125; X16973;
XX
DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector lambda ZAP Express - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pJF3 from pUC19 & T7 oligo
RC   pBS or BlueScribe from pJF3 & T3 oligo
RC   pBS+, pBS- from pBS or BlueScribe & pEMBL8
RC   pBluescript KS(+) from pBS+ & linker
RC   pBluescript KS(-) from pBS- & linker
RC   pBST-B from pBS & pEMBL8
RC   pBSITO#12 from pBST-B & pEMBL8
RC   pPreB from pBSITO#12 & pBluescript SK(-)
RC   lambda cI857 Sam7, lambda cI857 Sam100 nin5 from lambda
RC   lambda LongC from lambda cI857 Sam7 & pUC19 & lambda cI857 Sam100 nin5
RC   lambda LongD from lambda LongC
RC   lambda ZAP from lambda L47.1 & pPreB & lambda LongD
RA   Short J.M., Fernandez J.M., Sorge J.A., Huse W.D.;
RT   "Lambda ZAP: a bacteriophage lambda expression vector with in vivo
RT   excision properties";
RL   Nucleic Acids Res. 16:7583-7600(1988).
XX
RN   [2]
RC   pBK-RSV from pBR322 & RSV
RC   pBK-CMV from pBR322 & CMV
RC   lambda ZAP Express from lambda & pBK-CMV
RA   Alting-Mees M.A.;
RT   ;
RL   Strategies 5:58-61(1992).
XX
RN   [3]
RC   pBK-RSV from pBR322 & RSV
RC   pBK-CMV from pBR322 & CMV
RC   lambda ZAP Express from lambda & pBK-CMV
RA   Alting-Mees M.A., Vaillancourt P., Short J.M.;
RT   ;
RL   Plasmids A Practical Approach 0:0-0(1993).
XX
CC   The cI857 repressor is temperature sensitive and so is inactive
CC   at 42 degrees but active at 32 degrees, allowing selection against
CC   lysogeny.
CC   Contains phagemid pBK-CMV.
CC   NM (lambda ZAP Express)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (Stratagene)
CC   HO (E.coli XL-1-Blue MRF')
CC   CP ()
CC   FN (cloning up to 12000 bp)(cloning cDNA)(cloning unidirectional)
CC   FN (expression prokaryote)(expression eukaryote)(excision automatic)
CC   SE (color blue/white)
CC   PA (lambda)
CC   BR (lambda ZAP II)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   2. CMV
FT                   -> pBK-CMV
FT                   1. lambda
FT                   2. pBK-CMV
FT                   -> lambda ZAP Express"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosL)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda A"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda J"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda bio"
FT   misc_binding    0..0
FT                   /note="SIT NsiI"
FT   promoter        complement(0..0)
FT                   /note="PRO CMV immediate-early"
FT   misc_binding    0..0
FT                   /note="SIT NheI"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacUV5 gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    0..0
FT                   /note="MCS SacI-EcoRI-KpnI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI (in C-terminus of lacZ)"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="SIT SV40 3' splice site"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 polyA site"
FT   misc_binding    0..0
FT                   /note="SIT MluI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1-"
FT   misc_binding    0..0
FT                   /note="SIT SspI"
FT   misc_feature    0..0
FT                   /note="TER helper phage initiator"
FT   misc_feature    0..0
FT                   /note="TER helper phage terminator"
FT   misc_binding    0..0
FT                   /note="SIT NaeI"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT SspI"
FT   misc_binding    0..0
FT                   /note="SIT MstII"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   polyA_signal    0..0
FT                   /note="PLA mammal thymidine kinase polyA site"
FT   misc_binding    0..0
FT                   /note="SIT MstII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda shndIII2-3"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda cI857"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda nin5"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda S100 amber (Sam100)"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosL)"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//