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ID   LAMZAPII   preliminary; circular DNA; SYN; 1 BP.
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AC   IG2040; X04125; X16973;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda ZAP II - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pJF3 from pUC19 & T7 oligo
RC   pBS or BlueScribe from pJF3 & T3 oligo
RC   pBS+, pBS- from pBS or BlueScribe & pEMBL8
RC   pBluescript KS(+) from pBS+ & linker
RC   pBluescript KS(-) from pBS- & linker
RC   pBST-B from pBS & pEMBL8
RC   pBSITO#12 from pBST-B & pEMBL8
RC   pPreB from pBSITO#12 & pBluescript SK(-)
RC   lambda cI857 Sam7, lambda cI857 Sam100 nin5 from lambda
RC   lambda LongC from lambda cI857 Sam7 & pUC19 & lambda cI857 Sam100 nin5
RC   lambda LongD from lambda LongC
RC   lambda ZAP from lambda L47.1 & pPreB & lambda LongD
RA   Short J.M., Fernandez J.M., Sorge J.A., Huse W.D.;
RT   "Lambda ZAP: a bacteriophage lambda expression vector with in vivo
RT   excision properties";
RL   Nucleic Acids Res. 16:7583-7600(1988).
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RN   [2]
RC   Uni-ZAP XR from lambda ZAP II, EcoRI-XhoI
RA   Huse W.D., Hansen C.;
RT   ;
RL   Strategies 1:1-3(1988).
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CC   The cI857 repressor is temperature sensitive and so is inactive
CC   at 42 degrees but active at 32 degrees, allowing selection against
CC   lysogeny.
CC   Contains phagemid Bluescript SK-.
CC   NM (lambda ZAP II)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (Stratagene)
CC   HO (E.coli XL-1-Blue MRF')
CC   CP ()
CC   FN (cloning up to 10000 bp)(cloning cDNA)(cloning unidirectional)
CC   FN (expression prokaryote)(antibody screening)(excision automatic)
CC   SE (color blue/white)
CC   PA (lambda)
CC   BR (lambda ZAP Express)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda cI857 Sam7
FT                   1. lambda
FT                   -> lambda cI857 Sam100 nin5
FT                   1. lambda cI857 Sam7
FT                   2. pUC19
FT                   3. lambda cI857 Sam100 nin5
FT                   -> lambda LongC
FT                   1. lambda LongC
FT                   -> lambda LongD
FT                   1. lambda L47.1
FT                   2. pPreB
FT                   3. lambda LongD
FT                   -> lambda ZAP
FT                   1. lambda ZAP
FT                   2. lambda gt10, S+ gene
FT                   -> lambda ZAP II"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosL)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda A"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda J"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacUV5 gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    0..0
FT                   /note="MCS SacI-NotI-XbaI-SpeI-EcoRI-XhoI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI (in C-terminus of lacZ)"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   misc_feature    0..0
FT                   /note="TER helper phage initiator"
FT   misc_feature    0..0
FT                   /note="TER helper phage terminator"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1-"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda shndIII2-3"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda cI857"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda nin5"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda S100 amber (Sam100)"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosL)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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