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ID   LAMZIP     preliminary; circular DNA; SYN; 43900 BP.
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AC   IG1019;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda ZipLox - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [lambda gt7-lac5 from lambda gt7 & lac gene]
RC   phage from lambda gt7-lac5 & lambda 540
RC   lambda gt11 from phage & lambda gt4
RA   Young R.A., Davis R.W.;
RT   "Efficient isolation of genes by using antibody probes";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:1194-1198(1983).
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CC   NM (lambda ZipLox)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (BRL)
CC   HO (E.coli Y1088)(E.coli Y1089)(E.coli Y1089r-)
CC   HO (E.coli Y1090)(E.coli Y1090r-)
CC   CP ()
CC   FN (cloning cDNA)(fragment cloning up to 10000 bp)
CC   FN (fusion)(screening antibody)
CC   SE (color blue/white)
CC   PA (lambda)
CC   BR ()
CC   OF ()
CC   OR (E.coli Y1090)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda end-EcoRI 21227bp 1..21227, left arm
FT                   2. lambda gt11 remove XhoI-SalI(2) 260bp 31500..31760
FT                   SalI(1) <31500..<31500
FT                   fill in
FT                   SstI-end 18300bp 25400..43700, right arm
FT                   3. pZL1 loxP-loxP
FT                   EcoRI linker;SstI linker
FT                   -> lambda ZipLox 43900bp
FT                   \ [lambda lacOPZ' cIts857 nin5 Sam100]"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cos)"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda A"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda J"
FT   CDS             complement(0..0)
FT                   /note="REP E. coli lacI repressor gene"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    23570..23665
FT                   /note="MCS Sse6387I-PstI-KpnI-EcoRI-PinAI-RsrII-Kpn2I-
FT                   EcoRI-SmaI-SalI/AccI-SstI-SpeI-NotI-XmaIII-XbaI-BamHI-
FT                   HindIII-SnuBI-SunI-MluI-SphI-AatII,
FT                   in C-terminus of lacZ"
FT   misc_binding    23570..23665
FT                   /note="MCS unique EcoRI-SalI-SstI-SpeI-NotI-XbaI,
FT                   in C-terminus of lacZ"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacUV5"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage Sp6"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda incA"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda shndIII2-3"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda cI857"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda nin5"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda S100 amber (Sam100)"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cos)"
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SQ   Sequence 249 BP; 63 A; 67 C; 61 G; 58 T; 0 other;
     agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctaatac
     gactcactat agggaaagct ggtacgcctg caggtaccgg tccggaattc ccgggtcgac
     gagctcacta gtcggcggcc gctctagagg atccaagctt acgtacgcgt gcatgcgacg
     tcatagctct tcttatagtg tcacctaaat tcaattcact ggccgtcgtt ttacaacgtc
     gtgactggg
//