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ID   M13BLA6    preliminary; circular DNA; SYN; 8130 BP.
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AC   ATCC37039;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector M13bla6 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [M13::Tn3-15 from M13 & Tn3]
RC   M13bla6 from M13 & M13::Tn3-15
RC   M13bla cat1 from M13bla6 & pACYC184
RA   Hines J.C., Ray D.S.;
RT   "Construction and characterization of new coliphage M13 cloning
RT   vectors";
RL   Gene 11:207-218(1980).
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CC   Medium is 1592 SM buffer.
CC   NM (M13bla6)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteria-free phage preparation)(E.coli LA6)(E.coli K37)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (M13)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M13 HaeII 6407bp 5572..5572,
FT                   \ between genes II & IV
FT                   2. Tn3 BspHI-BspHI 1008bp 3898..4906,
FT                   \ amp gene/#V00613
FT                   -> M13::Tn3-15 7415bp
FT                   1. M13 HaeII 6407bp 5572..5572,
FT                   \ between genes II and IV
FT                   2. M13::Tn3-15 HaeII-HaeII 1720bp, B fragment amp gene
FT                   -> M13bla6 8130bp [unique PstI,XorII; HincII,amp/M13]"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI-XorII"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
FT   CDS             1..861
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 861 BP; 223 A; 203 C; 222 G; 213 T; 0 other;
     atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     tcactgatta agcattggta a
//