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ID   M13BLACAT1 preliminary; circular DNA; SYN; 9880 BP.
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AC   ATCC37040;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector M13bla cat1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [M13::Tn3-15 from M13 & Tn3]
RC   M13bla6 from M13 & M13::Tn3-15
RC   M13bla cat1 from M13bla6 & pACYC184
RA   Hines J.C., Ray D.S.;
RT   "Construction and characterization of new coliphage M13 cloning
RT   vectors";
RL   Gene 11:207-218(1980).
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CC   Medium is 1592 SM buffer.
CC   NM (M13bla cat1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteria-free phage preparation)(E.coli LA6)(E.coli K37)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (M13)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M13bla6 HaeII 8130bp 5572..5572, 5' to amp
FT                   2. pACYC184 HaeII-HaeII 1461bp 3369..4245..585, cat
FT                   -> M13bla cat1 9880bp [unique EcoRI in cat]"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//