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ID   M13JN72    preliminary; circular DNA; SYN; 480 BP.
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AC   M62983;
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DT   08-APR-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector M13JN72 - incomplete, MCS.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-480
RC   M13A4E from M13 & linker A4E
RC   M13JN10 from M13mp19 & linker
RC   M13JN22 from M13JN10 & pBR345
RC   M13JN32 from M13JN22
RC   M13JN66 from M13WB3295 & linker
RC   M13JN83 from M13WB3295 & linker
RC   M13JN72 from M13JN66
RC   M13JN95 from M13JN83
RA   Johnson D.F., Nierlich D.P., Lusis A.J.;
RT   "Use of the lac repressor in constructing sequential deletions and a
RT   new sequecing vector";
RL   Gene 94:9-14(1990).
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CC   NCBI gi: 208816
CC   NM (M13JN72)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (M13)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M13 Sau96I/AsuI 6407bp 5725..5725,
FT                   \ minus-strand ori
FT                   2. E. coli Sau96I-Sau96I 465bp, lac
FT                   -> M13WB2341 6872bp
FT                   1. M13WB2341 remove ClaI-ClaI 2895bp 6041..6407..2529,
FT                   \ 3512bp
FT                   2. M13mp9 ClaI-ClaI 2895bp 7233..7599..2529
FT                   -> phage 6407bp [no BamHI]
FT                   1. phage remove HindIII-EcoRI 30bp, MCS/M13 6605..6635
FT                   \ 6377bp
FT                   2. M13mp11 HindIII-EcoRI 45bp 6235..6280, MCS
FT                   -> M13WB3295 6422bp
FT                   1. M13mp19 remove AvaI-AvaI 444bp 5826..6270, 6806bp
FT                   fill in
FT                   HindIII linker 6bp aagctt
FT                   -> M13JN10 6810bp
FT                   1. M13JN10 EcoRI 6810bp, MCS/M13mp19 6286
FT                   2. pBR345 EcoRI-EcoRI, lac operator
FT                   -> M13JN22
FT                   1. M13JN22 EcoRI
FT                   Ethidium bromide
FT                   BAL31 nuclease
FT                   -> M13JN32
FT                   1. M13WB3295 HpaII 6422bp
FT                   Ethidium bromide
FT                   S1 nuclease
FT                   HindIII linker 6bp aagctt
FT                   -> M13JN66 6400bp
FT                   1. M13JN66 large HindIII-BglI
FT                   BAP
FT                   2. M13JN32 small HindIII-BglI
FT                   -> M13JN72 6834bp"
FT   misc_binding    83..140
FT                   /note="MCS from M13mp18"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
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SQ   Sequence 480 BP; 103 A; 138 C; 129 G; 110 T; 0 other;
     atttgggtga tggttcacgt agtgggcagt gagcgcaacg caattaatgt gagttagctc
     actcattagg cacccagctt tgcaagcttg catgcctgca ggtcgactct agaggatccc
     cgggtaccga gctcgaattc aaattgtgag cggataacaa tttgggccgt cgttttacaa
     cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatcccccc
     ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gccttcccaa cagttgcgca
     gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct
     ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc
     acggttacga tgcgcccatc tacaccaacg tgacctatcc ccctgataga cggtttttcg
//