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ID M13K07 preliminary; circular DNA; SYN; 8700 BP.
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE E. coli phage vector M13K07 - incomplete.
KW cloning vector.
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
RC M13KO7 from M13
RA Vieira J., Messing J.;
RT "Production of single-stranded plasmid DNA";
RL Meth. Enzymol. 153:3-11(1987).
CC There is some instability of the insert during growth if not
CC propagated correctly. (Poornima Dwivedi)
CC This a helper bacteriophage for production of single-stranded DNA from
CC plasmids containing M13 intergenic region which preferentially
CC packages single-stranded plasmid DNA over phage DNA. (ATCC staff)
CC Restriction digests analyzed on agarose gels give the following sizes
CC (kb): BamHI--8.7; HindIII--8.7. (ATCC staff)
CC Medium is 1592 SM buffer.
CC NM (M13K07)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phage)
CC SP (ATCC)
CC HO (bacteria-free phage preparation)(E.coli JM101)(E.coli MV1184)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
FH Key Location/Qualifiers
FT misc_feature 0..0
FT /note="1. M13 6407bp
FT 2. E. coli, kan gene
FT -> M13KO7 8700bp"
FT rep_origin 0..0
FT /note="ORI bacteriophage M13"
FT CDS 0..0
FT /note="ANT E. coli kanamycin resistance gene (kan)"
SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;