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ID   M13K07     preliminary; circular DNA; SYN; 8700 BP.
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AC   ATCC37468;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector M13K07 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   M13KO7 from M13
RA   Vieira J., Messing J.;
RT   "Production of single-stranded plasmid DNA";
RL   Meth. Enzymol. 153:3-11(1987).
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CC   There is some instability of the insert during growth if not
CC   propagated correctly. (Poornima Dwivedi)
CC   This a helper bacteriophage for production of single-stranded DNA from
CC   plasmids containing M13 intergenic region which preferentially
CC   packages single-stranded plasmid DNA over phage DNA. (ATCC staff)
CC   Restriction digests analyzed on agarose gels give the following sizes
CC   (kb): BamHI--8.7; HindIII--8.7. (ATCC staff)
CC   Medium is 1592 SM buffer.
CC   NM (M13K07)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteria-free phage preparation)(E.coli JM101)(E.coli MV1184)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. M13 6407bp
FT                   2. E. coli, kan gene
FT                   -> M13KO7 8700bp"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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