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ID   M13MP714     preliminary; circular DNA; SYN; 363 BP.
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AC   M29480;
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DT   15-MAR-1990 (Rel. 5, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   E.coli phage M13mp7-14 - incomplete, yeast DNA/pJD221 5'.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-363
RC   M13mp7-14+, M13mp7-14- from M13mp7 & pJD14
RA   Lescure B.;
RT   "Pure yeast RNA polymerase B (II) initiates transcription at
RT   specific points on supercoiled yeast DNA";
RL   J. Biol. Chem. 258:946-952(1983).
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RN   [2]
RC   from pJD14
RA   Lescure B., Williamson V., Sentenac A.;
RT   "Efficient and selective initiation by yeast RNA polymerase B in a
RT   dinucleotide-primed reaction";
RL   Nucleic Acids Res. 9:31-45(1981).
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RN   [3]
RC   from pJD221 & oligo
RC   pYecyc1-9 from CYC1 gene
RA   Lescure B., Bennetzen J., Sentenac A.;
RT   "In vitro transcription of the yeast alcohol dehydrogenase I gene
RT   by homologous RNA polymerase B (II). Selective initiation and
RT   discontinuous elongation on a supercoiled template";
RL   J. Biol. Chem. 256:11018-11024(1981).
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RN   [4]
RC   pY9T6 from yeast ADH1 gene
RC   pJD14 from pBR322 & pY9T6, yeast ADH1 gene
RA   Williamson V., Bennetzen J., Young E.T., Nasmyth K., Hall B.D.;
RT   "Isolation of the structural gene for alcohol dehydrogenase by
RT   genetic complementation in yeast";
RL   Nature 283:214-216(1980).
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RN   [5]
RC   pJD221 from pBR322 & pJD14
RA   Ammerer G.;
RT   ;
RL   Unpublished (1981).
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CC   NCBI gi: 209593
CC   NM (M13mp7-14)
CC   CM (no)
CC   NA (filamentous ss-DNA)(ds-DNA)
CC   TP (linear)(circular)
CC   ST ()
CC   TY (phage)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (yeast)(pJD221)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. yeast, ADH1 gene
FT                   -> pY9T6
FT                   1. pBR322 BamHI
FT                   2. pY9T6 BamHI-BamHI, yeast ADH1 gene
FT                   -> pJD14
FT                   1. M13mp7 BamHI
FT                   2. pJD14 BamHI-BamHI, yeast ADH1 gene
FT                   -> M13mp7-14+"
FT   misc_recomb     157..158
FT                   /note="S.cerevisiae DNA end/pJD221 DNA start"
FT   mRNA            313..>363
FT                   /note="RNA unidentified mRNA"
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SQ   Sequence 363 BP; 86 A; 83 C; 55 G; 139 T; 0 other;
     tcggcgtggt tgtgcaattg cgtaaactcg ttttttcggc gccgcaaagc caaatacatc
     atatcaacac ttttcacttt atttttcgtt cgacccttat atttgtcttt tgccttcatg
     ctccttgatt tcctatttca tttaccatca tttcttcgat ccccctcatc gtgatgtagt
     caaccctctt cttgttttaa tacgttaatc tccctgaaat tactttaaat acgtatgtgc
     gcaagtgcta gaaacatgta tacatcgagt atatatatgt atgtattcgc ttgatgtggg
     ttctggttat ctatacctta ttgattacat gcacacgcac gattacaatg accctttgta
     aca
//