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ID   M13SV8     preliminary; circular DNA; SYN; 66 BP.
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AC   M24175;
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DT   15-DEC-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector M13.SV.8 - incomplete, SV40 early promoter.
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KW   cloning vector; artificial gene; early promoter.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-66
RC   pMKD52 from pSV1
RC   M13.SV.8 from M13.TG.103 & pSV1
RC   M13.SV.23 from pSV1 & pMKD52
RC   M13.TG.103 from M13; [Transgene S.A.]
RC   M13.SV.B11, M13.SV.B12 from M13.SV.8 & M13.SV.23
RA   Everett R.D., Chambon P.;
RT   "A rapid and efficient method for region- and strand-specific
RT   mutagenesis of cloned DNA";
RL   EMBO J. 1:433-437(1982).
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RN   [2]
RC   pSV1 from pBR322 & SV40 early genes
RC   pAS from pSV1
RC   pEMP from pSV1 & SV40 T antigen
RC   HS0, HS1, HS2, HS3, HS4, HS6 from pSV1 & pEMP
RC   pHS102 from pBR322 & SV40
RA   Benoist C., Chambon P.;
RT   "Deletions covering the putative promoter region of early mRNAs of
RT   simian virus 40 do not abolish T-antigen expression";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:3865-3869(1980).
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CC   NCBI gi: 208806
CC   NM (M13.SV.8)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (M13.TG.103 from Transgene S.A.)(pSV1)
CC   BR (M13.SV.23)
CC   OF (M13.SV.B11)(M13.SV.B12)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 EcoRI 4361bp 4360..4360, ori/amp
FT                   DNA polymerase I
FT                   2. SV40 BamHI-HpaI 3211bp 2534..5243..502,
FT                   \ early region/0.73 to 0.15
FT                   DNA polymerase I:DNA polymerase I
FT                   -> pSV1 7572bp
FT                   1. M13.TG.103 remove EcoRI-HindIII 49bp,
FT                   \ MCS/M13mp2 6232..6881/7100bp
FT                   2. pSV1 HindIII-PvuII 344bp 5172..5243..273,
FT                   \ SV40 early promoter
FT                   -> M13.SV.8 7400bp"
FT   rep_origin      0..0
FT                   /note="ORI phage M13"
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SQ   Sequence 66 BP; 14 A; 32 C; 8 G; 12 T; 0 other;
     ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     cccagt
//