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ID   M13TG103   preliminary; circular DNA; SYN; 66 BP.
AC   K01678;
DT   15-JUN-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phage vector M13tg103 - incomplete, 5' end of lacZ gene.
KW   cloning vector; beta-galactosidase; galactosidase; lacZ gene.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-66
RC   M13tg103 from linker & M13mp701
RC   M13tg109 from linker & M13mp701
RC   M13tg110 from linker & M13mp701
RC   M13tg117 from M13tg110 & oligo
RC   M13tg114 from M13tg109 & oligo
RA   Lathe R.F., Kieny M.P., Schmitt D., Curtis P., Lecocq J.P.;
RT   "M13 bacteriophage vectors for the expression of foreign proteins in
RT   Escherichia coli: The rabies glycoprotein";
RL   J. Mol. Appl. Genet. 2:331-342(1984).
CC   [1] reports inserting a HindIII linker into the 5' end of the beta
CC   galactosidase gene of phage M13, so that controlled expression of
CC   inserted coding sequences could be obtained. The expression of
CC   viral proteins in M13 does not seem detrimental to the host, as it
CC   is in E.coli.
CC   NCBI gi: 209446
CC   NM (M13tg103)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (M13mp701)(M13)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. M13mp2 7196bp
FT                   -> M13mp5 7200bp
FT                   1. M13mp5 7200bp
FT                   -> M13mp701 7200bp [no HindIII]
FT                   1. M13mp701 AccI 7200bp, MCS/M13mp2 6881..6881
FT                   Klenow
FT                   HindIII linker 10bp ccaagcttgg
FT                   -> M13tg103 7200bp"
FT   misc_recomb     33..34
FT                   /note="M13mp701 DNA end/HindIII linker DNA start"
FT   misc_recomb     43..44
FT                   /note="HindIII linker DNA end/M13mp701 DNA start"
FT   CDS             1..>66
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   NCBI gi: 209447"
SQ   Sequence 66 BP; 15 A; 22 C; 15 G; 14 T; 0 other;
     atgaccatga ttacgaattc cccggatccg tcgccaagct tggcgacctg cagcaattca