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ID   M13TG114   preliminary; circular DNA; SYN; 60 BP.
AC   K01680;
DT   15-JUN-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phage vector M13tg114 - incomplete, 5' end of lacZ gene.
KW   cloning vector; beta-galactosidase; galactosidase; lacZ gene.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-60
RC   M13tg103 from linker & M13mp701
RC   M13tg109 from linker & M13mp701
RC   M13tg110 from linker & M13mp701
RC   M13tg117 from M13tg110 & oligo
RC   M13tg114 from M13tg109 & oligo
RA   Lathe R.F., Kieny M.P., Schmitt D., Curtis P., Lecocq J.P.;
RT   "M13 bacteriophage vectors for the expression of foreign proteins in
RT   Escherichia coli: The rabies glycoprotein";
RL   J. Mol. Appl. Genet. 2:331-342(1984).
CC   [1] reports inserting a HindIII linker into the 5' end of the beta
CC   galactosidase gene of phage M13, so that controlled expression of
CC   inserted coding sequences could be obtained. The expression of
CC   viral proteins in M13 does not seem detrimental to the host, as it
CC   is in E.coli. 8 bp (the SalI site) were deleted from M13tg114,
CC   destroying the BamHI site.
CC   NCBI gi: 209448
CC   NM (M13tg114)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (M13mp107)(M13)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. M13mp2 7196bp
FT                   -> M13mp5 7200bp
FT                   1. M13mp5 7200bp
FT                   -> M13mp701 7200bp [no HindIII]
FT                   1. M13mp701 BamHI 7200bp, MCS/M13mp2 2221..2221
FT                   Klenow
FT                   HindIII linker 10bp ccaagcttgg
FT                   -> M13tg109 7200bp
FT                   1. M13tg109 remove PstI-BamHI 8bp, MCS
FT                   2. oligo PstI-BamHI 8bp gatctgca
FT                   -> M13tg114 7200bp [HindIII, no BamHI, no SalI]"
FT   misc_recomb     28..29
FT                   /note="M13mp701 DNA end/synthetic linker 1 start"
FT   misc_recomb     38..39
FT                   /note="synthetic linker 1 end/synthetic linker 2
FT                   start"
FT   misc_recomb     46..47
FT                   /note="synthetic linker 2 end/M13mp701 DNA start"
FT   CDS             1..>60
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   NCBI gi: 209449"
SQ   Sequence 60 BP; 15 A; 18 C; 13 G; 14 T; 0 other;
     atgaccatga ttacgaattc cccggatccc aagcttggga tctgcagcaa ttcactggcc