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ID   M13TG115   preliminary; circular DNA; SYN; 66 BP.
AC   K01156;
DT   01-AUG-1984 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phage vector M13tg115 - incomplete, 5' end of lacZ gene.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-66
RC   ptg130 from pBR322 & oligo
RC   M13tg130 from M13tg109 & ptg130
RC   M13tg115 from linker & M13tg110
RC   M13tg119 from M13tg115 & linker
RC   M13tg120 from M13tg119 & M13mp701 & M13mp7
RC   M13tg131 from M13tg130 & M13tg120
RA   Kieny M.P., Lathe R.F., Lecocq J.P.;
RT   "New versatile cloning and sequencing vectors based on bacteriophage
RT   M13";
RL   Gene 26:91-99(1983).
CC   NCBI gi: 209450
CC   NM (M13tg115)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (M13)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. M13mp2 7196bp
FT                   -> M13mp5 7200bp
FT                   1. M13mp5  7200bp
FT                   -> M13mp701 7200bp [no HindIII]
FT                   1. M13mp701 SalI 7200bp, MCS
FT                   fill in
FT                   HindIII linker 10bp ccaagcttgg
FT                   -> M13tg110 7200bp
FT                   1. M13tg110 EcoRI 7200bp, M13mp2 6232..6232
FT                   S1 nuclease
FT                   2. BglII linker 8bp cagatctg
FT                   -> M13tg115 7200bp"
FT   CDS             1..>66
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   NCBI gi: 209451"
SQ   Sequence 66 BP; 15 A; 22 C; 15 G; 14 T; 0 other;
     atgaccatga ttacgcagat ctgccccgga tccgtccaag cttggacctg cagcaattca