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ID   M13TG117   preliminary; circular DNA; SYN; 63 BP.
AC   K01679;
DT   15-JUN-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phage vector M13tg117 - incomplete, 5' end of lacZ gene.
KW   cloning vector; beta-galactosidase; galactosidase; lacZ gene.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-63
RC   M13tg103 from linker & M13mp701
RC   M13tg109 from linker & M13mp701
RC   M13tg110 from linker & M13mp701
RC   M13tg117 from M13tg110 & oligo
RC   M13tg114 from M13tg109 & oligo
RA   Lathe R.F., Kieny M.P., Schmitt D., Curtis P., Lecocq J.P.;
RT   "M13 bacteriophage vectors for the expression of foreign proteins in
RT   Escherichia coli: The rabies glycoprotein";
RL   J. Mol. Appl. Genet. 2:331-342(1984).
CC   [1] reports inserting a HindIII linker into the 5' end of the beta
CC   galactosidase gene of phage M13, so that controlled expression of
CC   inserted coding sequences could be obtained. The expression of
CC   viral proteins in M13 does not seem detrimental to the host, as it
CC   is in E.coli. The spacing between EcoRI and BamHI sites was
CC   increased by 1 bp from that in clone M13tg110, producing a SmaI
CC   site.
CC   NCBI gi: 209452
CC   NM (M13tg117)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (M13mp107)(M13)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. M13mp2 7196bp
FT                   -> M13mp5 7200bp
FT                   1. M13mp5 7200bp
FT                   -> M13mp701 7200bp [no HindIII]
FT                   1. M13mp701 AccI 7200bp, MCS/M13mp2 6881
FT                   S1 nuclease
FT                   HindIII linker 10bp ccaagcttgg
FT                   -> M13tg110 7200bp
FT                   1. M13tg110 remove EcoRI-BamHI, 7200bp
FT                   2. oligo EcoRI-BamHI 11bp aattccccggg
FT                   -> M13tg117 7200bp [HindIII, BamHI, EcoRI]"
FT   misc_recomb     15..16
FT                   /note="M13mp701 DNA end/synthetic linker start"
FT   misc_recomb     25..26
FT                   /note="synthetic linker end/M13mp701 DNA start"
FT   misc_recomb     32..33
FT                   /note="M13mp701 DNA end/ HindIII linker DNA start"
FT   misc_recomb     42..43
FT                   /note="HindIII linker DNA end/M13mp701 DNA start"
FT   CDS             1..>63
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   NCBI gi: 209453"
SQ   Sequence 63 BP; 15 A; 19 C; 15 G; 14 T; 0 other;
     atgaccatga ttacgaattc ccggggatcc gtccaagctt ggacctgcag caattcactg